目的：探讨肿瘤新生微血管密度(microvessel density,MVD)与原发性肝细胞癌(hepatocellular carcinoma,HCC)侵袭和转移的关系。 方法：采用免疫组化法(labelled streptavidin biotin,LSAB酶标记的链酶亲合素—生物素)对手术切除后30例原发性肝细胞癌和20例癌旁组织的石蜡包埋组织标本中的MVD进行了检测。在400倍视野下选择5个最密集区，计算MVD数量，取其均数。 结果：①MVD在HCC组织中比癌旁组织明显增高(P<0.01)；②在HCC中有转移者及包膜不完整者，MVD明显高于无转移者及包膜完整者(P<0.05)；③大肿瘤(直径>5 cm)与小肿瘤(直径≤5 cm)MVD比较无统计学意义(P>0.05)。 结论：原发性肝细胞癌组织中的MVD与肿瘤的侵袭和转移行为密切相关，可作为判定HCC生长、转移及预后的指标。
Objectives:To study the relationship between tumor MVD and invasion/metastasis of primary hepatocellular carcinoma(HCC). Methods:Paraffin-embedded specimens from tumor tissue in 30 patients with primary hepatocellular carcinoma and liver tissue in 20 ones who had undergone radical hepatectomy were studied. Angiogenesis was assessed by the microvessel density(MVD) method using LSAB immunohistochemical staining. The mean of MVD were counted in the five areas of highest density under 400 times magnification microscopy. Results:①The MVD in HCC was higher than that in the non-tumorous liver tissue(P<0.01).②Metastatic tumor MVD or noncapsulated tumor MVD were higher than those without metastasis or well-encapsulated tumors(P<0.05).③There was no significant difference between large HCC(diameter >5 cm) MVD and small HCC(diameter≤5 cm) MVD(P>0.05). Conclusions:MVD are highly correlated with invasion/metastasis of primary HCC, which may serve as a parameter for determining tumor biology, metastasis and prognosis, also in supervising and treating HCC.
journal of Medical Postgraduate yixue yanjiusheng xuebao
Primary hepatocellular carcinoma