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罗汉果组培苗二次脱毒技术研究 预览 被引量:2

Study on Duplicate Virus-free Techniques of Siraitia grosvenorii Tissue Culture Seedling
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摘要 对罗汉果(Siraitia grosvenorii)组培苗采用热处理和剥取茎尖技术进行二次脱毒处理,脱毒效果采用间接酶联免疫法和电镜观察法进行检测。结果表明,适合材料热处理的培养基为MS+0.3 mg/L 6-BA+NAA 0.1 mg/L+0.1 mg/L IBA,热处理材料的顶芽呈锥形,利于剥取茎尖;茎尖分化最佳培养基为MS+0.3 mg/L 6-BA+0.05 mg/L NAA+0.1 mg/L GA3;38.5℃热处理10 d剥取长1.0~1.5 mm的茎尖进行二次脱毒培养,存活率为80.00%,脱毒率为100.00%。 The tissue culture seedlings of Siraitia grosvenorii were treated by heat treatment and tip-stripping to obtain virus-free seedling.ELISA(enzyme-linked-immunosorbent serologic assay) method and electron microscope method were applied for virus detection.The results showed that the suitable medium for heat treatment was MS+0.3 mg/L 6-BA+0.1 mg/L NAA+0.1 mg/L IBA.Terminal bud after heat treatment was conical and easy for stripping stem tip.The optimum medium for inducement of tips was MS+0.3 mg/L 6-BA+0.05 mg/L NAA+0.1 mg/L GA3.Heat treatment for 10 d at 38.5 ℃ twice,stripping shoot-tip for 1.0~1.5 mm long,the survival rate was 80.00%;and the detoxication rate was 100.00%.
作者 吴群英 李伯林 李景云 WU Qun-ying1,LI Bo-lin2,LI Jing-yun3(1.Biotechnology College,Guilin Medical University,Guilin 541004,Guangxi,China; 2.College of Life Science,Guangxi Normal University,Guilin 541004,Guangxi,China; 3.College of Basic Medicine,Guangxi University of Chinese Medicine,Nanning 530001,China)
出处 《湖北农业科学》 北大核心 2013年第5期1185-1187,共3页 Hubei Agricultural Sciences
基金 广西科技攻关项目(桂科攻0630002-3F)
关键词 罗汉果(Siraitia grosvenorii)花叶病毒 茎尖 热处理 脱毒 酶联免疫法 Siraitia grosvenorii virus(WMV-2-Luo) shoot-tip heat treatment virus-free ELISA
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