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小麦萌发素G和ψG的纯化及其部分特性 预览 被引量:4

Purification and Some Characteristics of Germins Gand ψG from Wheat
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摘要 利用亲和层析方法纯化了小麦草酸氧化酶G和ψG,并对其生化特性进行了初步分析。G和ψG的最适pH为3.5,在60℃以下较稳定。当草酸浓度大于0.2mmol/L时,G和ψG的活性受到抑制。G和ψG的Km值分别为0.084和0.053mmol/L。0.1mmol/L的EDTA、NH4^+、Cl^-、Mn^2+、Mg^2+、Na^+和K^+对G和ψG的活性没有影响,0.1mmol/L的Zn^2+、Cu^2+、Fe^2+、Al^3+、CO3^2-、NO3^-和SO4^2-抑制G和ψG的活性。0.1mmol/L的H2PO4^-和HPO4^2-仅抑制G的活性.0.1mmol/L的核黄素、FMN和FAD抑制ψG活性,G的活性则不受FAD和FMN的影响。 By affinity chromatography, germins G and ψG were purified from roots of wheat seedling and wheat embryos imbibition for 4 h (Table1). Characterization of the germins G and ψGwere studied.Theresults showed that Gand ψG were stable at temperatures lower than 60℃ (Fig.4), and had an optimum pH at 3.5 (Fig.1). The Km value of G for oxalate was 0.084 mmol/ L, while that of ψG was 0.053 mmol/L (Fig.3). Oxalate showed substrate inhibition effect above 0.2 mmol/Lon G and ψG (Fig.2). EDTA, NH4 , + Mn2+, Mg2+, Na+ and K+ at a concentration of 0.1 mmol/L had no effect on OxO activity. CO3 , NO3 and SO4 2- - 2- at a concentration of 0.1 mmol/L inhibited partially the activities of G and ψG, 0.1mmol/L Cu2+, Fe2+, Al3+ completely in- hibited the activities of G and ψG, but addition of 0.1 mmol/L EDTA partially relieved the inhibition, which indicated that metal cations may make the oxalate unavailable by chelating it. H2PO4 and HPO4 inhibited the activity of -2-G, but had no effect on ψG. Riboflavin, FMN and FAD inhibited ψG, but FMN and FAD had no effect on G (Table 2).
作者 刘娥娥 郭振飞 LIU E-E, GUO Zhen-Fei* (College of Life Sciences, South China Agricultural University, Guangzhou 510642, China)
出处 《植物生理与分子生物学学报》 CAS CSCD 2004年第4期 393-398,共6页 Journal Of Plant Physiology and Molecular Biology
基金 广东省自然科学基金项目(No. 000594)资助。~~
关键词 小麦 萌发素 草酸氧化酶 纯化 特性 wheat germin oxalate oxidase purification characterization
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  • 1Aguilar C, Urz úa U, Koening C, Vicufna R (1999). Oxalate oxidase from Ceriporiopsis subvermispora: biochemical and cytochemical studies. Arch Biochem Biophys, 366 (2):275-282 被引量:2
  • 2Berna A, Beemier F (1997). Regulation expression of a wheat germin gene in tobacco: oxalate oxidase activity and apoplastic localization of the heterologous protein. Plant Mol Biol, 33:417-429 被引量:2
  • 3Chitiboga (1966). Purification and properties of oxalic acid oxidase. Arch Biochem Biophys, 116:516-523 被引量:1
  • 4Dumas B, Sailland A, Cheviet JP, Freyssinet G, Pallett K (1993).Identification of barley oxalate oxidase as a germin-like protein. CR Acad Sci Paris, 316:793-798 被引量:1
  • 5Grzelczak ZF, Lane BG (1984). Signal resistance of a soluble protein to enzymeic hydrolysis. An unorthodox approach to the isolation and purification of germin, a rare growthrelated. Can J Biochem Cell Biol, 62:1351-1353 被引量:1
  • 6Jaikaran ASI, Kenedy TD, Dratewka-kos E, Lane BG (1990).Covalently bonded and adventitious glycans in germin. J Biol Chem, 265:12503-12512 被引量:1
  • 7Kotsira VP, Clonis YD (1997). Oxalate oxidase from barley roots: purification to homogeneity and study of some molecular, catalytic and binding properties. Arch Biochem Biophys, 340(2):239-249 被引量:1
  • 8Kotsira VP, Clonis YD (1998). Chemical modification of barley root oxalate oxidase shows the presence of a lysine,a carboxylate, and disulfides, essential for enzyme activity.Arch Biochem Biophys, 356(2): 117-126 被引量:1
  • 9Lane BG, Grzelezak ZF, Kennedy TD, Kajioka R, Orr J,D’Agostino S, Jaikaran A (1986). Germin:compartmentation of two forms of the protein by washing growing wheat embryos. Biochem Cell Biol, 64:1025-1037 被引量:1
  • 10Lane BG, Cuming AC, Fregean J, Carpita NC, Hurkman WJ,Bemier F, Dratewka-kos E, Kennedy TD (1992). Germin isoforms are discrete temporal markers of wheat development. Pseudogermin is a uniquely thermostable water-soluble oligomeric protein in ungerminated 被引量:1

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