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菊属野生种、栽培菊花及种间杂种的RAPD分析 预览 被引量:35

RAPD analysis of wild species,cultivars and interspecifichybrids in Dendranthema
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摘要 以菊属野生种、栽培菊花及种间杂种为试材,采用RAPD方法研究其亲缘关系.从40条随机引物中筛选出15条,对23份试材进行扩增,共产生153条带,平均每条引物扩增出10.2条带,多态性条带占77.1%.通过遗传相似性矩阵及UPGMA聚类分析,结合杂交结实性与幼胚拯救成苗率,认为:父母本亲缘关系近的遗传距离较小,杂交亲和性好,结实率较高,幼胚拯救易成功;反之杂交亲和性差,难以结实,幼胚拯救成苗率低.根据以上数据结合前人研究结果,对栽培菊花的起源演化进行了探讨. The relationship of 23 wild species,cultivars and interspecific hybrids in Dendranthema was studied by RAPD analysis.15 random primers were screened to detect polymorphism among the total materials used.Of the 153 bands produced,77 1% were polymorphic.Each primer amplified an average of 10 2 bands.The results from pairwise similarity and UPGMA cluster analysis,combining with the fecundity of hybridization and rate of plantlet of embryo rescue,suggested that when parents have closer relationship,the genetic distance was closer,the compatibility of hybridization was better,fecundity was higher and embryo rescue was easier.When parents have further relationship,the results were contrary.According to the RAPD analysis and previous research,the origin and evolution of Dendranthema morifolium was discussed.
作者 李辛雷 陈发棣 LI Xin lei,CHEN Fa di * (College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)
出处 《南京农业大学学报》 CAS CSCD 北大核心 2004年第3期 29-33,共5页 Journal of Nanjing Agricultural University
基金 江苏省应用基础研究计划项目,江苏省高校高新技术产业发展项目,江苏省科技攻关项目,江苏省高技术研究发展计划项目
关键词 菊属 野生种 栽培 菊花 种间杂种 杂交亲和性 亲缘关系 Dendranthema RAPD phylogeny compatibility of hybridization embryo rescue
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