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甘薯茎尖分生组织培养与快速繁殖技术研究 预览 被引量:12

Research on Stem-apex Meristem Culture and Rapid Reproduction Technology of Sweet Potato
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摘要 甘薯茎尖分生组织培养与快速繁殖技术研究课题己进行3a,筛选出最佳培养基为MS+6-BA1.0mg/L+NAA0.1~0.2mg/L+3%蔗糖+琼脂6.0g/L;最适宜培养温度为26~30℃;症状学诊断法、指示植物鉴定法进行病毒检测,平均脱毒率达83.7%.脱毒试管苗切段快速增殖,繁殖系数为5n,繁殖速度以几何级数增长,一定时间内可大量繁殖脱毒苗.脱毒苗移栽大田进行产量比较,平均增产率达37.8%. The three-year research on stem-apex meristem culture and fast reproduction technology of sweet potato selected out MS+6-BA 1.0mg/L+NAA 0.1~0.2mg/L+3%sucrose+agar6.0g/L and 26~30℃ respectively as the preferential medium and appropriate temperature range. And the average devirus rate was 83.7% based on the semiological diagnostic and plant indicator identification. The reproduction coefficient for the fast propagation with cut test-tube plantlets was 5 n, the reproduction speed increased at a geometric progression and large-scale seedlings could be multiplied in a short time. The virus-free seedlings transplanted in fields reached a yield increase of 37.8%.
作者 关崇梅 秦静远 徐志英 刘玉凤 张西丽 龙金娟 Guan Chongmei,Qin Jinyuan,Xu Zhiying,Liu Yufeng,Zhang Xili,Long Jinjuan(Yangling Vocational and Technical College,Yangling,Shaanxi 712100)
出处 《中国农学通报》 CSCD 2004年第4期 33-35,共3页 Chinese Agricultural Science Bulletin
基金 杨凌职业技术学院校科研和教改项目
关键词 甘薯 茎尖 分生组织培养 快繁技术 培养基 Sweet potato stem-apex Meristem culture Rapid reproduction
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