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荧光偏振法测定荧光素的荧光寿命的研究 预览 被引量:1

STUDY OF FLUORESCENCE POLARIMETRIC METHOD FOR DETERMINATION OF SODIUM FLUORESCEIN LIFETIME
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摘要 用荧光偏振法测定荧光素的荧光寿命的灵敏度和准确度较高。在荧光素钠荧光强度最强时-激发波长490nm,发射波长515nm、pH值8.5、溶液浓度1.6×10^-5mol/L,测定荧光素钠溶液的荧光偏振度,测定不同温度时荧光素钠溶液的粘度,再根据Perrin公式,求出荧光素钠的荧光寿命。用荧光偏振法测得荧光素钠荧光寿命为3.4ns、p<0.02,与文献值符合良好,结果令人满意。 A simple, fast and relatively highly accurate method for the determination of the lifetime of sodium fluorescein is presented in the paper. Under the condition ofλ_(ex)=490nm, λ_(em) 515nm, c=1.6×10~(-5)mol/L and pH=8.5, the fluorescent polarity of sodium fluorescein solution was measured with fluorescence polarimetric method. The measured result of sodium fluorescein lifetime was calculated according to Perrin formula is 3.4ns, p<0.02, it is in accordance with the value in the literature and the result is satisfactory.
作者 张宝棣 王继波 ZHANG Baodi, WANG Jipo, LIU Yiulan Department of Chemistry, Qingdao Medical College
出处 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 1993年第6期 113-116,共4页 Spectroscopy and Spectral Analysis
关键词 荧光偏振法 荧光素钠 荧光寿命 Fluorescence polarimetric method, Sodium fluorescein, Fluorescent, lifetime
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