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乙脑病毒CN株PrM/E基因的克隆及序列分析 预览 被引量:3

Cloning and Sequencing of PrM/E Genes of Japanese Encephalitis Virus (JEV) CN Strain
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摘要 通过RT-PCR扩增乙型脑炎病毒CN株主要抗原基因PrM/E,并将PrM/E基因克隆、测序后,与Genban K发表的JEV SA14(U14163)基因序列进行比较分析,发现CN株与JEV SA14强毒株的同源性为98%.在JEV基因组5′端477~2477的长2000nt的决定JEV抗原性的PrM/E区中,CN株与SA14株有40个碱基不同,其中28个碱基突变为同义突变,另外12个为错义突变,导致相应的氨基酸序列(666个)中的4个氨基酸发生了突变,其中有2个氨基酸出现在含有关键的抗原决定簇的E蛋白内,但不影响其功能表达. This paper reports the cloning of the PrM/E gene of the Japanese Enceplialitis Virus (JEV) CN strain by RT-PCR and analysis of the gene sequence. Comparison of the gene sequence with that of the JEV SA14 strain demonstrates 98% sequence homology. In the 2?Kb genome region of the virus, between nucleotides 477~2477, 40 base pairs differe between the two strain. Only 12 of these differences are missense mutations that result in 4 amino acid differences in the protein sequences. Two of the amino acid substitutions occur in the region encoding for the E protein and do not cause a change in the activity of this protein between the two strains of JEV.
作者 汤德元 郭万柱 徐志文 颜其贵 王印 王晓玉 TANG De-yuan~1,2, GUO Wan-zhu~1, XU Zhi-wen~1, Yan Qi-gui~1, Wang Yin~1, Wang Xiao-yu~1(1.College of Animal and Technology, Sichuan Agricultural University, Yaan 625014, Sichuan, China;2.College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, China)
出处 《四川农业大学学报》 2004年第3期 252-256,共5页 Journal of Sichuan Agricultural University
关键词 日本乙型脑炎病毒 PrM/E基因克隆 核酸序列 Japanese encephalitis virus PrM/E genes cloning sequence analysis
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