Objective To investigate the effects of immune tolerance induced by peptide based on nucleosome H2B epitopes in prevention and treatment of systemic lupus erythematosus (SLE). Methods Based on a model of experimental systemic lupus erythematosus induced with syngeneic apoptotic lymphocytes to BALB/c mice, immune tolerance was induced by H2B14~28, administration intravenously with H2B Th cell epitope peptide. The effects of prevention and treatment were evaluated by changes in autoantibodies and SLE-like clinical manifestations. Results The peptide of H2B14~28 was highly effective in preventing autoantibody production of SLE (such as ANA, anti-dsDNA Abs, anti-RNP Abs and ACL Abs), and ameliorating clinical manifestations. Conclusion Tolerance to nucleosome induced by H2B14~28 peptide can significantly inhibit autoantibodies production and down-regulate clinical manifestations of SLE. So it may be a new way to treat and prevent SLE by nucleosome-based immune tolerance vaccine.
Chinese Journal of Rheumatology