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宫内感染致早产鼠脑瘫动物模型制备及其鉴定的实验研究 预览 被引量:62

A study on establishing a new preterm animal model with cerebral palsy and identifying the animal model
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摘要 目的:探索宫内感染致早产鼠脑性瘫痪动物模型的制备方法,并对脑瘫动物模型进行鉴定.方法:脑瘫动物模型的制备:给予16只孕16、17天的实验组大鼠脂多糖每次350μg/g,连续2天腹腔注射,8只对照组孕鼠腹腔注射同剂量的生理盐水.观察两组孕鼠的分娩时间及新生仔鼠出生体重.孕22天前分娩的仔鼠为早产鼠.将实验组中提前分娩的母鼠和对照组母鼠处死后,取子宫和胎盘HE染色观察.模型鉴定:①神经行为学检测:30日龄早产鼠16只,足月产鼠30只,分别进行随意运动障碍、姿势异常、肌张力异常、不自主动作、情感行为能力及倾斜板试验,记录各项评分并进行统计学处理;②病理学检测:30日龄早产鼠16只,足月产鼠16只,断头取脑,光镜与电镜下观察脑组织变化.结果:①实验组有6只大鼠提前分娩,共获活早产仔鼠26只;对照组均为足月分娩,产仔鼠74只.早产仔鼠出生后颜色青紫、活动少、体重低;②子宫及胎盘病理检测可见提前分娩母鼠子宫壁及胎盘内血管充血、水肿,并见大量中性粒细胞浸润;③16只30日龄早产鼠中,10只经鉴定为脑瘫鼠.④脑瘫大鼠随意运动障碍,姿势、肌张力异常,病理结果显示脑损伤部位在白质,系胶质细胞、髓鞘的损伤.结论:①孕鼠腹腔注射LPS可诱发早产;②LPS致宫内感染可成功制备早产鼠脑瘫动物模型;③脑瘫鼠具有神经行为学异常;④脑瘫鼠动物模型的病理改变以白质损伤为主. Objective:To establish a new animal model with cerebral palsy and identify the animal model.Method: 24 Wistar pregnant rats were divided into two groups: lipopolysaccharide(LPS) (n=16) and normal saline(n=8). Pregnant rats were consecutively injected intraperitoneally with LPS (350μg/kg) or saline on gestation 16d and 17d. The interval from infusion to delivery for each group was recorded, as well as their birth weight. The abilities of sensorimotor movement (hanging test and inclined plane test),emotional behavior(open field test and resistant to capture test), posture, and muscle tension, et al were tested till 30d. The brain damage of the preterm rats was observed by optical and electron microscopes.Result:There were 26 live preterm pups delivered by 6 LPS-treated rats.The birth weight of the pups reduced compared with the saline controls.And there was motor reduction of preterm pups.There was intrauterine infection of LPS group rats.There was white matter damage of animal modal. 10 of 16 preterm pups were the ones with cerebral palsy.The behavioral abilities of CP pups were much lower than those of normal control group.There was much abnormality of posture and muscle tension with CP pups.Conclusion: Preterm delivery can be induced by LPS.A new animal model for investigation of cerebral palsy is established by LPS-induced preterm.A method for identifying rats with cerebral palsy is successfully established. The damage location of the CP animal model is white matter.
作者 李晓捷 高晶 孙忠人 LI Xiaojie, GAO Jing, SUN Zhongren College of Rehabilitation Medicine, Jiamusi University, Jiamusi,154002
出处 《中国康复医学杂志》 CAS CSCD 2004年第12期 885-889,共5页 China Journal of Rehabilitation Medicine
关键词 早产 脑瘫 动物模型 仔鼠 宫内感染 对照组 孕鼠 自主 结论 实验 intrauterine infection preterm cerebral palsy animal model identification
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参考文献16

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