Bovine mammary epithelial cell lines were obtained by culturing tissue fragment.Then the appearance of these cells were studied by light microscope.The results are as follows:The fibroblast cell and the bovine mammary epithelial cells grow in different areas.The bovine mammary epithelial cells were cultured in vitro through single-cell afloat,which formed island monolayer aggregates,then the cells propagated and formed dome-like construction.The cell nucleus is circular or oval with 2-4 cores.In the culture medium with estrogen,the nucleus type changed.The mammary epithelial cells can be developed into many cell types,the majority of which are short shuttle-like or guboidal.The cells connected with each other properly and formed net-like construction.
Journal of Northwest Sci-Tech Univ of Agr and
in vitro culture
mammary epithelial cell
the appearance of cells