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灰背栎遗传多样性和遗传结构的AFLP指纹分析 预览 被引量:8

AFLP Analysis of Genetic Variation and Structure of Natural Populations of Quercus senescens
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摘要 用AFLP方法对灰背栎(Quercus senescens)8个居群进行了遗传多样性、居群遗传结构研究.TFPGA软件分析两组引物组合共产生125个位点,其中94个为多态位点,多态位点百分率为75.2%,发现灰背栎居群的遗传变异水平有随着海拔升高而遗传多样性下降的趋势.Arliquin 2.000中的AMOVA分析表明灰背栎居群间分化大,分化指数达φst=0.2956.用PAUP软件对所有个体间的遗传关系进行了聚类分析. The population variation and structure of 115 plants representing 8 populations of Quercus senescens Hand.-Mazz. were investigated by amplification fragment length polymorphism (AFLP) analysis.Using TFPGA software,94 polymorphic phenotypic patterns (75.2%) was obtained using 2 AFLP primer combinations and it was found that genetic variation of different population was negatively correlated with altitudes.Analysis of the genetic structure of populations carried out using analysis of molecular variation (by Arliquin 2.000 software),It showed that there was a high significant population differentiation (φ_(st)=0.2956).And neighbour-joining dendragram also be constructed based on 2 primer combinations by PAUP software.
作者 王淑霞 胡运乾 周浙昆 WANG Shu-Xia~(1,2),HU Yun-Qian~1,ZHOU Zhe-Kun~2(1 Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,China;2 Graduate School of the Chinese Academy of Sciences,Beijing 100039,China)
出处 《云南植物研究》 CSCD 北大核心 2005年第1期49-58,共10页 Acta Botanica Yunnanica
基金 国家自然科学基金,国家重点基础研究发展计划(973计划)
关键词 灰背栎 AFLP 遗传多样性 遗传结构 纵向岭谷区 Quercus senescens Amplified fragment length polymorphism(AFLP) Genetic variation Genetic structure Longitudinal Range-Gorge
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