期刊文献+

去甲斑蝥素诱导小鼠肺纤维瘤L929细胞凋亡 预览 被引量:24

Apoptosis of L929 cells induced by norcantharidin
在线阅读 下载PDF
分享 导出
摘要 目的:研究去甲斑蝥素(norcantharidin,NCTD)抑制小鼠肺纤维瘤(L929)细胞的作用机制.方法:用MTT法测定细胞生长抑制率.采用Hoechst 33258染色、DNA凝胶电泳和乳酸脱氢酶(LDH)活力检测细胞凋亡.用免疫印迹法(Western blot)分析了细胞外信号调节激酶(extracellular signal-regulated kinase,ERK)和磷酸化ERK、c-Jun N-末端激酶(C-Jun N-terminal kinase,JNK)和磷酸化JNK蛋白表达的变化.结果:NCTD诱导L929细胞凋亡.JNK抑制剂(SP600125)与ERK抑制剂(PD98059),可明显抑制NCTD对细胞的杀伤作用.同时磷酸化ERK和磷酸化JNK表达增加.结论:NCTD对L929细胞的细胞毒作用是通过诱导其凋亡而发生的,且呈时间剂量依赖性.这种作用可能与JNK,ERK通路激活有关. Purpose:To study the mechanism of norcantharidi n (NCTD)-induced apoptosis on L929 cell lines. Methods:Inhibitory effects of NCTD on L929 cell lines were meas ured by MTT test. Morphological changes were observed by Hoechst 33258 staining. DNA gel electrophoresis and LDH activity test were used to determine apoptosis. The mechanism of NCTD-induced apoptosis was analyzed by addition of several ki nds of mitogen activated protein kinase (MAPK) inhibitors and by Western blot an alysis. Results:NCTD induced L929 cell death in a time and dose depende nt manner. The cytotoxic effect of NCTD on L929 cell lines were induced mainly t hrough apoptotic pathways. Membrane blebbing and chromatin condensation were obs erved in NCTD-treated L929 cells. DNA fragments were also detected by agarose g el electrophoresis. By adding of MAPK (JNK or ERK) inhibitors, a distinct inhibi tion of apoptosis was detected. Western blot analysis showed that the expression of ERK proteins was decreased whereas the expression of phosphorylated form of ERK was increased depending on the time period. At the same time, the expression of JNK showed no change, while the expression of phosphorylated JNK was increas ed. Conclusions:The cytotoxic effect of NCTD on L929 cell lines was mainly due to apoptosis. ERK and JNK MAPKs might be involved in the apoptotic p athways of NCTD-treated L929 cells.
作者 安巍巍 薛莲 王敏伟 田代真一 小野寺敏 池岛乔 AN Wei-wei, XUE Lian, WANG Min-wei, et al (China-Japan Research Institute of Medical and P harmaceutical Sciences, Department of Pharmacology, Shenyang Pharmaceutical Univ ersity, Shenyang Liaoning 110016, China)
出处 《中国癌症杂志》 CAS 2005年第1期 22-25,共4页 China Oncology
关键词 小鼠肺纤维瘤 L929细胞 去甲斑蝥素 凋亡 细胞外信号调节激酶 C-JUN N-末端激酶 mouse fibrosarcoma L929 cells norcantharidin apoptosis ERK JNK
  • 相关文献

参考文献10

  • 1孙震晓,李家实.去甲斑蝥素抗肿瘤研究热点[J].西北药学杂志,1998,13(5):227-229. 被引量:38
  • 2朱立平,陈学清主编..免疫学常用实验方法[M],2000:467.
  • 3Hong CY, Huang SC, Lin Sze-K, et al. Norcantharidin-induced post - G2/M apoptosis is dependent on wild-type 1:63 gene[ J].Biochem. Biophys Res Commun,2000,276( 1 ) :278-285. 被引量:1
  • 4Humphreys DT, Wilson MR. Modes of L929 cell death induced by TNF-alpha and other cytotoxic agents [ J ]. Cytokine, 1999,11(10) : 773-782. 被引量:1
  • 5Hernnann M, Lomz HM, Voll R, et al. A rapid and simple method for the isolation of apoptotic DNA fragments [ J ]. Nucleic Acids Res, 1994,22( 24 ) :5506-5507. 被引量:1
  • 6Charrier L, Jorry A, Toquet C, et al. Growth phase-dependent expression of ICAD-L/DFF45 modulates in human colonic cancercells [ J ]. Cancer Res, 2002,62 ( 7 ) : 2169-2174. 被引量:1
  • 7Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual [ M ] 2nd ed. NY Cold Spring Harbor Laboratory Press, 1989:880-898. 被引量:1
  • 8Los M, Mozoluk M, Ferrari D, et al. Activation and caspase-mediated inhibition of PARP: a molecular switch between fibroblast necrosis and apoptosis in death receptor signaling [ J ]. Mol Biol Cell, 2002,13(3) : 978-988. 被引量:1
  • 9Strelow A, Bemardo K, Adam-Klages S, et al. Overexpression of acid ceramidase protects from tumor necrosis factor-induced cell death[J]. J Exp Med, 2000,192(5) : 601-612. 被引量:1
  • 10Gardners AM, Johnson GL. Fibroblast growth factor-2 suppression of tumor necrosis factor or-mediated apoptosis requires Ras and the activation of mitogen-activated protein kinase [ J ]. J Biol Chem, 1996,271 ( 24 ) : 14560-14566. 被引量:1

二级参考文献7

共引文献37

同被引文献328

引证文献24

二级引证文献113

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈