A two-stage culture method was established to culture Arnebia euchroma (Royle) Johnston cells in suspension and 5L outloop airlift bioreactor. Relationship between cell growth, shikonin formation and changes of conductivity, soluble sugar in cultured medium were studied. At the first stage, cells grew very fast, with the formation of a small amount of shikonin derivatives, and conductivity and soluble sugar decreased sharply. After cultured the cells in the second stage, conductivity decreased at first, and then grew up after a large amount of shikonin derivatives formed and secreted into the medium. By minotoring the changes of conductivity and soluble sugar, cell growth and shikonin derivatives formation were estimated in situ during the large-scale culture of A. euchroma cells.
Chinese Journal of Biotechnology
Arnebia euchroma (Royle) Johnston, suspension culture, bioreactor culture, conductivity, soluble sugar