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江苏省现阶段麻疹流行株H1基因型 被引量:5

H1 Genotype of the Current Circulating Strain of Measles Virus in Jiangsu Province
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摘要 目的监测现阶段在江苏省流行的麻疹野病毒及其基因型别.方法 2003年5月分别在医院门诊疑似麻疹病人和疑似麻疹爆发点的病人中采集了咽拭子,接种EB病毒转化的绒猴淋巴母细胞(B95a细胞),获得3株麻疹野病毒,同时进行了血球凝集(HA)试验、血球吸附试验.结果该3株病毒均无HA和血球吸附活性,逆转录-聚合酶链反应和其产物的基因序列分析提示,这3株病毒属于麻疹野病毒第8基因组H1基因型.结论此次为江苏省首次分离到麻疹野病毒,并证实在江苏省流行的麻疹野病毒基因型与国内流行优势基因型相同. Objective To survey the current circulating strain of wild measles virus in Jiangsu Province and its genotype. Methods In May 2003,the swab samples of the suspected measles patients in hospital were collected and measles outbreak spot were suspected,inoculated into B95a cell derived from EB virus and got 3 strains of wild measles virus,at the same time,the hemagglutination(HA)test and hemadsorption test were conducted. Results The results showed that these 3 strains of measles viruses had no HA and hemadsorption activity.RT-PCR and the sequence analysis of its product suggested that these 3 strains of measles viruses were belong to the H_1 genotype of eight genetic group of wild measles virus. Conclusion The wild measles virus were isolated for the first time in Jiangsu Province and this demonstrated that the genotype of wild measles virus circulating in Jiangsu was the same as the dominant genotype circulating in China.
作者 张晋琳 赵红 范刚 汤奋扬 马福宝 张燕 许文波 ZHANG Jin-lin,ZHAO Hong,FAN Gang,et al.(Center for Disease Control and Prevention of Jiangsu Province,Nanjing 210009,China)
出处 《中国计划免疫》 2005年第1期19-20,共2页 Chinese Journal of Vaccines and Immunization
关键词 麻疹野病毒 血球凝集试验 血球吸附试验 逆转录一聚合酶链反应 基因组(型) Wild measles virus Hemagglutination test Hemadsorption test RT-PCR Genotype Genetic group
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