Objective To survey the current circulating strain of wild measles virus in Jiangsu Province and its genotype. Methods In May 2003,the swab samples of the suspected measles patients in hospital were collected and measles outbreak spot were suspected,inoculated into B95a cell derived from EB virus and got 3 strains of wild measles virus,at the same time,the hemagglutination(HA)test and hemadsorption test were conducted. Results The results showed that these 3 strains of measles viruses had no HA and hemadsorption activity.RT-PCR and the sequence analysis of its product suggested that these 3 strains of measles viruses were belong to the H_1 genotype of eight genetic group of wild measles virus. Conclusion The wild measles virus were isolated for the first time in Jiangsu Province and this demonstrated that the genotype of wild measles virus circulating in Jiangsu was the same as the dominant genotype circulating in China.
Chinese Journal of Vaccines and Immunization