期刊文献+

MNNG诱发新生大鼠腺胃癌模型的建立及其在胃癌发… 预览 被引量:21

Induction of gastric adenocarcinoma in Wistar rats following neonatal expesure to MNNG and neoplastic transformation assay of DNA of the induced cancer
在线阅读 下载PDF
分享 导出
摘要 通过给新生Wistar大鼠连续10次(每日1次)1-甲基-3硝基-1亚硝基胍(MNNG)灌胃,成功地诱发出腺胃癌、腺瘤和异型增生等病变。在0.4mg/只剂量组,这三种病变的诱发率分别为39%、50%和100%,腺胃癌的诱发率与MNNG剂量成正比,雄性大鼠高于雌性(P<0.02),70%病变发生在胃窦部。本模型具有致癌物用量小、易定量、给药期短、省时省力和不易污染环境等优点。研究还发现6个腺胃癌中有 denocarcinoma, adenoma and dysplasia of the stomach of adult Wistar rats were induced following administration of MNNG for 10 days by gavage when they were new一born. The incidence of the three types of pathological lesions at the dose of 0.4mg MNNG per rat being 39%,50%and 100%respectively.Induction of gast ric adenocarcinoma is dose一dependent. The incidence of adenocarcinoma in male rats being significantly higher then that of female rats(P<0.02). 23 of 33(70%)induced cancers were found in the gastric antrum. By the use of DNA cotransfection technique and the assay of carcinogenicity in the Balb/c nude mice, it was also found that the DNA of 4of the 6 induced gastric carcinomas could transform NIH /3T3 cells into malignant cells,an indication that the induced cancer DNA contains transforming gene.
作者 邓大君 朱少侠 Deng Da-jun,Zhu Shao-xia, Chen Qiang et al Department of Cancer Etiology, Beijing Institute for Cancer Research and College of Clinical Oncology,keijing Medical University,Beijing 100034
出处 《中华病理学杂志》 CAS 北大核心 1994年第5期 293-295,T058,共4页 Chinese Journal of Pathology
基金 国家自然科学基金
关键词 疾病模型 腺癌 胃肿瘤 Disease models,animal Adenocarcinoma
  • 相关文献

参考文献1

同被引文献204

引证文献21

二级引证文献56

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈