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食管癌和贲门癌组织中P53基因突变的研究 预览 被引量:19

MUTATION OF p53 GENE IN HUMAN CANCERS OF THE ESOPHAGUS AND GASTRIC CARDIA
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摘要 应用核酸杂交、PCR扩增、直接测序等分子生物学技术,分析了林县原发性食管癌和贲门癌及其癌旁组织中抑癌基因P53人子结构改变。用Southem杂交法分析的35例食管鳞癌和27例组织中,有1例P53基因缺失,1例重排结构性改变,占7.4%。上述食管鳞癌标本,用PCR直接测序,发现50%915/30)的食管鳞癌有P53基因的的5-9外显子和相应内含突变。45%(5/11)食管癌旁组织中有P53基因突变。 Abstract p53 gene in human esophageal cancer (EC) and cancer of gastric cardia was analyzed. South-ern blotting hybridization revealed that five of 35 of EC sample were found to contain abnormal structure of p53 gene, including 2 deletions and 3 rearrangements; two of 27 adjacent non-tumortissues also contain abnormal structure of p53 gene(7.4%),among them one case was fragementdeletion and another case was rearrangement. PCR-direct sequencing technique was used to de-tect p53 point mutation within exon and intron 5 through 9.Fifteen of 30(50%)of esophagealsquamous cell carcinomas contained mutation of p53 gene. Five of 11(45%)adjacent non-tumor tissues also contained mutation of p53 gene. An esophageal adenocarcinoma showed p53 mutation.Three of 4 carcinoma of gastric cardia showed p53 mutation .Mutation spectrum in EC:8of 22 cases (36.4%)of p53 mutation were G.C to A: T transition, 6 of 22 cases (27.3%)ofp53 mutation were frameshift mutation,including 13.6%(3/22)insertion and 9.1%(2/22)deletionmutation. Some new sites of p53 mutation in human EC were identified.The results suggest that the p53 gene plays an important role in carcinogenesis of human esophagus and gastric cardia.
作者 李华川 陆士新 Li Hua-chuan, Lu Shi-xin Cancer Institute, Chinese Academy of Medical Sciences Peking Union of Medical College, Beijing 100021
出处 《中华肿瘤杂志》 CAS CSCD 北大核心 1994年第3期 172-176,T007,共6页 Chinese Journal of Oncology
基金 国家“八·五”科技攻关资助课题
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