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当归补血汤成分的LC-ESI/TOFMS分析 预览 被引量:9

Analysis of the Composition in Danggui Buxue Decoction by LC-ESI/TOFMS
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摘要 目的:采用液相色谱于飞行时间质谱仪联用技术(LC-TOFMS)对中药复方当归补血汤中成分进行分析,并对主要成分进行鉴别.方法:用Zorbax SB C18色谱柱,以0.1%甲酸水溶液(A)/甲醇(B)梯度洗脱,DAD检测波长280 nm,使用ESI离子源,正离子与负离子模式下采集数据.结果:可确定其准确分子量的化合物有27个,能正确鉴定的有15个,另发现7个多肽,给出了其准确分子量.结论:经液相色谱的分离,借助TOFMS测定的准确分子量及其它信息可以鉴别中药复方中的成分,为中药复方中成分的鉴定提供了一种快速准确的方法. AIM: Apply the techniques of liquid chromatography coupled with time of flight mass spectrometry by using electrspray ionization (LC-ESI/TOFMS) to analyze and identify the compositions of Danggni Buxue (Radix Asteris and Radix Angelicae sinensis) decoction. METHOD: The chromatographic conditions are as follows: Zorbax SB C18(250 mm× 4.6 mm, 5/μm) column; mobile phase: 0.1% formic acid-water (A) and methanol (B), gradient elute; wavelength: 280nm. Use the ESI source and acquire the data in positive and negative mode. RESULT: The accurate molecular weights of twenty-seven compounds were measured in Danggui Buxue decoction. Among them, fifteen compounds were identified. Furthermore, seven polypeptides were detected as well as the accurate molecular weights were measured. CONCLUSION: It is rapid and accurate method that the compositions of compound prescription of Traditional Chinese Medicine can be identified in terms of the separation of liquid chromatography, the accurate molecular weights measured by TOFMS and other information.
作者 宋越 盛亮洪 盛龙生 李萍 Song Yue;Cheng LiangHong;Cheng LongSheng;Li Ping
出处 《中国天然药物》 SCIE CAS CSCD 2005年第5期 298-302,共5页 Chinese JOurnal of Natural Medicines
基金 国家自然科学基金,国家自然科学基金
关键词 当归补血汤 成分分析 LC-ESI/TOFMS Danggui Buxue decoction Composition analysis LC-ESI/TOFMS
作者简介 李萍:教授,博导,Tel: 025-85391244 E-mail: lipingli@public1.ptt.js.cn 通讯作者
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