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丹参酮ⅡA对放射性肺纤维化防治作用的实验研究 预览 被引量:42

The protective effect of Tanshinone ⅡA in radiation-induced pulmonary fibrosis
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摘要 目的 探讨丹参酮ⅡA对分次照射放射性肺纤维化的防治效果及机制。方法 雌性Wistar大鼠,6MVX线右胸照射,3Gy/次,5次/周,总量30Gy。每次照射前1h腹腔注射丹参酮ⅡA磺酸钠15ms/ks。照射后5个月取大鼠肺组织进行HE染色、Masson染色、转化生长因子-β(TGF-β1)免疫组化染色、羟脯氨酸含量及实时荧光定量RT-PCR检测TGF-β1 mRNA的表达变化。结果 每克湿肺羟脯胺酸含量在对照组、照射组与丹参酮加照射组分别为(2l,99±3,96)、(38,25±7.18)、(28,94±4.29)pg/g。照射组较对照组肺泡炎、纤维化病变加重,羟脯氨酸含量、TGF-β1蛋白及mRNA的表达升高;丹参酮加照射组较照射组肺泡炎、纤维化病变减轻,羟脯氨酸含量、TGF-β1表达下降。结论 丹参酮ⅡA能增加肺组织对放射性损伤的耐受,其机制可能通过抑制TGF-β1表达,使炎症及纤维化病变减轻。 Objective To investigate the protective effect and it' s possible mechanism of Tanshinone ⅡA in radiation-induced pulmonary fibrosis, Methods Having the right hemithorax of female Wistar rats irradiated 30 Gy in 10 fractions within 14 days by 6 MV photons, the radiation-induced pulmonary fibrosis animal model was established, In the treatment group, sodium Tanshinone ⅡA sulfonate ( 15 mg/kg) was given by intraperitoneal injection 1 hour before each fraction of irradiation, Five months after irradiation, the difference of the histopathological changes, the hydroxyproline content and expression of TGF-β1 between the radiation alone group, tanshinone plus radiation and control group were analyzed by HE stain, Massion stain, immunohistochemical methor and reverse transcriptase polymerase chain reaction(RT-PCR) method. Results The histopathological comparison revealed the protective effect of Tanshinone ⅡA. The content of hydroxypmline was (21.99 ± 3.96), (38.25 ±7.18), (28.94 ± 4.29) μg/g in the control group, radiation alone group and radiation plus Tanshinone n A. The expression of TGF-β1(mRNA and protein) was reduced by Tanshinane ⅡA. Pathological changes of the pulmonary fibrosis was reduced by Tanshinone ⅡA yet. Condusiom Our study shows that Tanshinone ⅡA can inhibit radiation-induced pulmonary fibrosis, and the possible mechanism of its may be made possible through down-regulating the expression of TGF-β1 in the irritated lung tissue.
作者 李广虎 李志平 徐泳许 峰王瑾 LI Guang-hu, LI Zhi- ping, XU Yong , XU Feng , WANG Jin, (Department of Radiation 0ncology , West China Hospital, Sichuan Univershy, Chengdu 610041, China)
出处 《中华放射肿瘤学杂志》 北大核心 2006年第1期 50-54,共5页 Chinese Journal of Radiation Oncology
关键词 丹参酮ⅡA 辐射防护剂 大鼠 Tanshinone ⅡA Radiation-protective agents Lung Rats
作者简介 通讯作者:李志平,Email:zhiping02442003@yahoo.com.con
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