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PP333与BA组合对怀地黄试管苗生长发育的影响 预览 被引量:6

The Influence of PP333 in Combination with Benzylaminopurine on Growth of Rehmannia glutinosa Plantlets
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摘要 本文研究TPP333与BA组合对怀地黄(Rehmannia glutinosa)试管苗生长发育的影响。结果表明:(1)与对照相比,不同浓度的PP333与0.5mg·L^-1BA组合均严重地抑制试管苗根、茎和叶的生长,表现为植株矮小,生根晚,前期根畸形生长(呈棒状或球状);(2)与单独使用0.5mg·L^-1BA相比,低浓度PP333(0.01和0.1mg·L^-1)与0.5mg·L^-1BA组合促进茎的伸长和加粗;(3)2mg·L^-1PP333与0.5mg·L^-1BA组合能显著地提高愈伤组织芽的分化率和腋芽的萌发率,有利于试管苗的快速繁殖。 The influence of PP333 with benzylaminopurine (BA) on growth of Rehmannia glutinosa plantlets was studied in this paper. Combinations of (0.5 mg·L^-1) BA and different concentrations of PP333 inhibited the growth of roots, stems and leaves significantly. Plantlets were short and small, and roots appeared later. The roots looked like clubs or balls initially. Compared with (0.5 mg·L^-1) BA, (0.5 mg·L^-1) BA with lower concentrations of PP333, at 0.01 or 0.1 mg·L^-1, improved the extension and thickness of stems. MS medium supplied with (2 mg·L^-1) PP333 and (0.5 mg·L^-1) BA increased the rate of bud formation of callus and pullulation of axillary buds observably, which is good for largescale in vitro propagation.
作者 李明军 徐鑫 夏民 张晓丽 柳俊 谢从华 Mingjun Li, Xin Xu, Min Xia, Xiaoli Zhang, Jun Liu, Conghua Xie (1 .College of Life Science, Henan Normal University, Xinxiang 453007; 2. Department of Biology, Nanyang Normal College, Nanyang 473000; 3. Key Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070)
出处 《植物学通报》 CSCD 北大核心 2006年第1期 56-59,共4页 Chinese Bulletin of Botany
基金 河南省重点科技攻关项目(0423032100)
关键词 怀地黄 试管苗 PP333 BA 生长发育 繁殖 Rehmannia glutinosa, plantlet, PP333, BA, growth, propagation
作者简介 通讯作者Author for correspondence.E-mail:limingjun2002@263.net
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