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慢性特发性血小板减少性紫癜患者Ⅰ类和Ⅱ类T细胞特点的研究 被引量:14

Profiles of type 1 and type 2 T cells in chronic idiopathic thrombocytopenic pnrpnra
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摘要 目的探讨慢性特发性血小板减少性紫癜(ITP)患者Ⅰ类和Ⅱ类T细胞的特点。方法采取30例慢性ITP患者外周血及8例慢性ITP患者和6例遗传性球形红细胞增多症患者的脾细胞(接受脾切除的患者),应用流式细胞仪技术检测辅助T细胞(Th)CD4^+’和细胞毒性T细胞(Tc1)CD8^+胞质内细胞因子干扰素γ(IFN-γ)/白细胞介素(IL)-4的比例;应用实时定量聚合酶链反应(PCR)检测T-bet和GATA-3 mRNA的表达水平。结果疾病活动期患者外周血Th1/Th2和Tc1/Tc2的比例(25.62和30.23)显著高于正常对照组(8.29和12.58,均P〈0.01);缓解期患者外周血Th1/Th2和Tc1/T02的比例(9.86和10.10)与正常对照组相比,差异无统计学意义。ITP患者脾细胞Th1/Th2的比例(41.46)显著高于对照组(16.30,P〈0.01);Tc1/T02的比例(35.80)高于对照组(16.88),但差异无统计学意义。疾病活动期患者外周血以及ITP患者脾细胞GATA-3mRNA水平明显低于对照组(是对照组的0.20和0.34倍,均P〈0.05),缓解期患者与对照组相比,差异无统计学意义(是对照组的0.97倍)。ITP患者外周血及脾细胞T-bet mRNA的表达水平与对照组相比,差异无统计学意义(是对照组的1.17和1.04倍)。结论ITP是Ⅰ类T细胞占优势的自身免疫性疾病。促进Ⅰ类T细胞模式向Ⅱ类T细胞转化可能为ITP患者提供一种潜在的免疫治疗方式。 Objective To explore the profiles of type 1 and type 2 T cells in chronic idiopathic thrombocytopenic purpura (ITP) patients. Methods Samples of peripheral blood were collected from 30 chronic ITP patients, 8 males and 22 females, aged 34, 20 being in the active stage, and 10 in the remission stage, and 20 healthy persons, 7 males and 13 females, aged 31. Peripheral blood mononuclear cells (PBMCs) were isolated, cultured, and activated with PMA/ionomycin. Flow cytometry was used to measure the intracellular cytokines interferon (IFN)-γ and interleukin (IL)-4 in the PBMCs so as to identify the Th1 cells (IFN-γ^+L-4^+ CD4^+ cells) , Th2 cells (IFN-γ^-L-4^+ CD4^+ cells), Tc1 cells ( IFN-γ^+L- 4^- CD8^+ cells), and Tc2 cells ( IFN-γ^- IL-4^+ CD8^+ ceils). The ratios of Th2/Tc2, Th1/Th2, and Tc1/ Tc2 were calculated. Samples of spleen tissue were collected from 8 patients with chronic ITP, 3 males and 5 females, aged 30, and 6 patients with hereditary splenocytosis (HS), 3 males and 3 females, aged 35, who underwent splenectomy. Splenocytes were isolated, cultured, and activated with PMA/ionomycin. Realtime PCR was used to detect the mRNA expression of T-bet and GATA-3 in the PBMCs and splenocytes. Results The Th1/Th2 ratio of the patients in active stage was 25.62, significantly higher than those of the patients in remission stage (9. 86) and the control (8. 29, both P 〈 0. 01 ), and the Tc1/Tc2 ratio of the patients in active stage was 30. 23, significantly higher than those of the patients in remission stage ( 10. 10) and the controls ( 12. 58, both P 〈 0. 01 ). The Th1/Th2 ratio of the splenocytes of the patients in active stage was 41.46, significantly higher than that of the controls ( 16. 30, P 〈0. 01 ), and the Tc1/Tc2 ratio of the splenocytes of the active ITP patients was 35. 80, not significantly higher than that of the controls (16. 30, P = 0. 082). The GATA-3 mRNA expression level of the PBMCs of the active ITP patien
作者 王婷婷 赵辉 任贺 郭建海 徐茂强 杨仁池 韩忠朝 WANG Ting-ting ,ZHAO Hui , REN He, GUO Jian-hai , XU Mao-qiang , YANG Ren-chi , HAN Zhong-chao(State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China)
出处 《中华医学杂志》 CAS CSCD 北大核心 2006年第10期 669-673,共5页 National Medical Journal of China
基金 人事部留学回国人员基金资助项目
关键词 紫癜 血小板减少性 特发性 T淋巴细胞 干扰素Ⅱ型 Purpura, thrombocytopenic, idiopathic T lympbocytes Interferon type Ⅱ
作者简介 通讯作者:杨仁池,Email:tcyang65@163.com
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参考文献15

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