期刊文献+

辽宁省乙脑病毒的分离与鉴定 预览 被引量:37

Isolation and Identification of Japanese encephalitis virus in Liaoning Province
在线阅读 下载PDF
分享 导出
摘要 目的 对2002年辽宁省采集的蚊虫标本进行病毒分离与鉴定。方法 从辽宁省采集蚊虫标本4927只,用细胞培养的方法分离病毒,对新分离的病毒进行血清学(ELISA和IFA方法)、分子生物学鉴定。结果 本实验共研磨蚊虫标本50批,分离到2株病毒,命名为LN02-102、LN02,104。这2株病毒均可致BHK-21细胞病变(CPE)(2—3d),致Vero细胞病变(2—4d),也可致C6/36细胞病变(2—4d)。对3日龄乳鼠2—3d可致死。这2株病毒可与标准乙脑病毒抗体反应。利用病毒PrM区段(基因组456-695核苷酸)进行基因分型分析。新分离的2株病毒属于基因Ⅰ型乙脑病毒。对这2株病毒的E基因区段进行分析,它们之间核苷酸和氨基酸的同源性为100%,与疫苗株SA14-14-2相比,核苷酸差异为4.11%,氨基酸差异在0.60%。结论 在辽宁省采集的蚊虫标本中分离到2株病毒,经血清学和分子生物学鉴定为基因Ⅰ型乙脑病毒,是近年来在辽宁省首次分离到基因Ⅰ型的乙脑病毒。 Objective To study arboviruses carried by mosquitoes in Liaoning Province in 2002. Methods Totally 4927 mosquitoes were collected from Liaoning Province in July 2002. Virus strains were isolated by inoculating BHK-21, C6/36 and Vero cells. The newly isolated strains were identified by serological (ELISA and IFA)and molecular methods (Real-Time PCR and RT-PCR). Results Two strains were isolated from mosquitoes causing cytopathogenic effect (CPE) on cells and were fatal, to suckling mice. Serological tests showed that both were positive for the antibody to JEV. The PrM and E gene were cloned and sequenced. The phylogenetic analysis showed that the new isolates belonged to genotype Ⅰ , JEV. Sequence analysis showed that the homology of nucleotide sequences and amino acid (AA) sequences between the two strains was 100%. Compared with the nucleotide sequences between the two strains and the standard JEV vaccine strain SA14-14-2, the difference was up to 4.11%, and the difference of AA was 0.6 %. Conclusion Two strains of JEV were isolated and identified in Liaoning province,both belonged to genotype Ⅰ JEV.
作者 王俊文 付士红 王环宇 毛晓燕 刘卫滨 何英 孙肖红 蔡增林 吴立平 赵习芳 韩瑞红 景雅 梁国栋 WANG Jun-wen, FU Shi- hong, WANG Huan-yu, MAO Xiao-yan, LIU Web-bin, HE Ying, Sun Xiao-hong, CAI Zeng-lin, WU Li-ping, ZHAO Xi-fang , Han Rui-hong , JING Ya , LIANG Guo-dong . (Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052 ,China)
出处 《中华实验和临床病毒学杂志》 CAS CSCD 北大核心 2006年第1期 61-65,F0003,共6页 Chinese Journal of Experimental and Clinical Virology
基金 科技部重大传染病专项(2003BA712A08-01)
关键词 脑炎病毒 日本 种系发生 基因 Encephalitis virus,Japanese Phylogeny Genes
  • 相关文献

参考文献15

二级参考文献65

共引文献150

同被引文献530

引证文献37

二级引证文献164

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈