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绵羊GnRHR基因部分序列PCR-SSCP分析 预览 被引量:7

PCR-SSCP analysis on partial sequence of gonadotropin releasing hormone receptor gene in sheep
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摘要 采用PCR—SSCP技术,用两对引物分析了GnRHR基因外显子2和外显子1部分序列在小尾寒羊、多赛特羊2个绵羊品种中的多态性。结果表明,对于引物1扩增片段,2个绵羊品种均只检测到AA基因型。对于引物2扩增片段,2个绵羊品种均检测到AA、AB基因型;小尾寒羊AA、AB基因型频率分别为0.83和0.17,多赛特羊AA、AB基因型频率分别为0.60和0.40。引物2的多态片段测序分析表明,位于GnRHR基因cDNA第230处发生了碱基的改变(G→C),该突变导致了氨基酸的改变(甘氨酸→半胱氨酸)。 The polymorphism of exon 2 and partial sequence of exon 1 of gonadotropin releasing hormone receptor (GnRHR) gene in Small Tail Han sheep and Dorset sheep was analyzed by PCR-SSCP. The results indicated that there was one genotype (AA) detected by primer 1 in two sheep breeds,and two genotypes (AA and AB) in Small Tail Han sheep and Dorset sheep detected by primer 2. In Small Tail Han sheep, frequencies of AA, AB genotypes were 0. 83,0. 17,respectively. In Dorset sheep,these frequencies were 0. 60,0. 40. The polymorphic fragment amplified by primer 2 was sequenced. The sequencing result showed that there was one nucleotide mutation ( G →C) at cDNA 230 of GnRHR gene in sheep, and this mutation resulted in amino acid change (Gly→Cys).
作者 刘忠慧 储明星 陈国宏 方丽 叶素成 LIU Zhong-hui, CHU Ming-xing, CHEN Guo-hong, FANG Li, YE Su-cheng ( 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009 ; 2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094)
出处 《安徽农业大学学报》 CAS CSCD 北大核心 2006年第3期 318-321,共4页 Journal of Anhui Agricultural University
基金 国家高技术研究发展计划(863计划)(2005AA211080)资助.
关键词 绵羊 促性腺激素释放激素受体基因 PCR—SSCP sheep gonadotropin releasing hormone receptor gene PCR-SSCP
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