Objective To establish the liver metastases of colonic adenocareinoma for experimental study of the effect of compound shenqitang in nude mice. Methods Nude mice liver metastasis model of colonic cancer was established with human colonic cancer calls line （ Ls 174t） inoculated into mice spleen. Granular forage with compound shenqitang was fed in experimental group and granular forage without compound shenqitang was fed in control group daily. The weight and size of the mice and growth of the carcinoma were recorded. All specimens were examined histologically. CK20 mRNA in blood in nude mice with liver metastasis of colonic carcinoma was detected with nested RT - PCR. Flesults The incidence of liver metastasis was 100 % in this intrasplenie injection model. The pathological results showed that tumour cells of liver metastases was poorly differentiated human colonic adenocareinoma. In experimental group, the tumor number and the weight of liver metastases were significantly lower than those in control group（ P 〈 0.05 ）. Using semiquantitative examination ,in experimental group the relative value of CK 20 mRNA expression in blood （0.40 ± 0.14 ） was significantly lower than that（ 0. 52 ± 0.09 ） in control group （ P 〈 0.05 ）. Conclusion Compound shenqitang could effectively inhibit the occurrence of liver metastasis carcinoma and decrease the positive expression of CK20 mRNA in experimental group, suggesting that compound shenqitang may become the potential therapeutic strategy for liver metatstasis of colonic cancer.
Journal of Yunyang Medical College