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血瘀状态下荷瘤小鼠肝转移的特点及相关机制研究 预览 被引量:5

The Effect of Syndrome on Tumor's Hepatic Metastasis
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摘要 目的:以荷B16黑色素瘤细胞的血瘀证小鼠为研究对象,观察血瘀状态下恶性肿瘤肝转移的特点。通过观察血瘀状态下荷瘤小鼠血清HGF水平变化。了解血瘀证影响恶-巨肿瘤肝转移的相关可能机制。方法:雌性C57BL/6J小鼠,20只,6—8周龄,随机分为2组。每组10只。单纯荷瘤组:以生理盐水股四头肌注射,每天1次,连续14天,第15天经脾接种B16黑色素瘤建立肝转移模型;血瘀荷瘤组:以利血平注射液股四头肌注射,每天1次,连续14天。第15天经脾接种B16黑色素瘤建立肝转移模型。建立肝转移模型第12天摘眼球取血,脱椎处死动物,观察肝转移情况以及测定血清HGF水平。结果:①肝转移阳性率:单纯荷瘤组为100%,血瘀荷瘤组为70%。两组相比无明显差异(P〉0.05)。②肝转移结节数及肝转移抑制率:血瘀荷瘤组肝转移结节数明显减少(P〈0.01)。肝转移抑制率为65.41%。③血清HGF水平:血瘀荷瘤组血清HGF水平明显低于单纯荷瘤组(P〈O.01)。结论:在血瘀状态下,HGF水平降低有可能影响肿瘤细胞在肝内的生长、运动、侵袭,最终使荷B16黑色素瘤小鼠肝转移减少。 Objective: to study the effect of of blood on tumor's hepatic metastasis, we duplicated stasis on the mice, Based on this model, we produced hepatic metastasis by intrasplenic injection into the mice of B16 Melanoma cells and observed the tumor's hepatic metastasis and serum HGF level. Methods: 20 C57BL/6J mice (male,6 -8weeks)were randomized into two groups equally. The mice in the non atasis group were injected N.S. into the quadriceps femoris once a day for 14 days,The mice in stasis group were injected reserpine into the quadriceps femoris once a day for 14 days. On day 15 ,we produced hepatic metastasis by intrasplenic injection into the mice of B16 Melanoma cells. On day 27, the blood was collected by removing the eye balls to detect the serum HGF level,we also observed the number of the hepatic metastasis. Results: (1)hepatic metastasis rate: Hepatic metastasis rate is 100% in non stasis group or 70% in stasis group. It was not statistically different ( P 〉 0.05) between two groups. (2)the number of hepatic metastsasis :The number of hepatic metastasis in stasis group was signiilcandy lower ( P 〈0.01 ) by 65.41% than that in stasis group. (3)serum HGF levellserum HGF level in Xue YuZheng group was markedly ( P 〈0.01) decreased as compared with non Xue YuZheng group. Conclusion : it seemed that the change of serum HGF level might be related to the effect of Xue YuZheng on tumor's hepatic metastasis.
作者 金莉 刘鲁明 JIN Li , LIU Lu-ming ( 1. Chinese Medicine Department, the First Hospital of Hangzhou City, Huangzhou 310006, Zhejiang, China; Chinese Medicine Department, Tumor Hospital Affiliated Fudan University, Shanghai 200032, China)
出处 《中华中医药学刊》 CAS 2007年第1期 75-78,共4页 Chinese Archives of Traditional Chinese Medicine
基金 国家中医药管理局基金资助(2000-J-P-34)
关键词 血瘀证 B16黑色素瘤 肝转移 HGF syngrome of blood stasis B16 Melanoma Hepatic Metastasis HGF
作者简介 金莉(1978-),女,浙江金华人,医师,硕士,研究方向:肿瘤的浸润与转移。
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