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高、低淋巴道转移能力小鼠肝癌细胞株抑制性消减杂交文库的构建 预览

Construction of Suppression Subtracted Hybridization (SSH) Library for Mouse Hepatocarcinoma Cell Line with High and Low Potential Lymphogenous Metastasis
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摘要 应用抑制性消减杂交(suppression subtracted hybridization,SSH)技术,分别构建2个不同淋巴道转移能力小鼠肝癌细胞株的SSH文库,高转移文库以高转移能力细胞株Hca-F为检测子(tester),同源的低转移能力细胞株Hca-P为驱赶子(driver);低转移文库则相反.随机筛选2个文库阳性克隆进行测序,并在GenBank数据库中进行同源性比较.成功构建高、低淋巴道转移能力小鼠肝癌细胞株SSH文库,高、低转移文库中分别包含995个及967个阳性克隆;其中,95%的阳性克隆含有300bp~1 000bp不等的插入片段;随机测序显示,文库中含有已知的基因、ESTs及与任何序列无同源性的新基因片段.
作者 崔晓楠 唐建武 侯力 宋波 班丽英 CUI Xiao-nan, TANG Jian-wu,HOU Li,et al.
出处 《中国肿瘤》 CAS 2007年第8期 620-624,共5页 Bulletin of Chinese Cancer
基金 国家自然科学资金项目(30500586)
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参考文献21

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