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苹果α-法尼烯合酶基因组结构和序列的多态性分析 预览 被引量:9

Genomic Organization and Sequence Polymorphism of E, E-alpha Farnesene Synthase Gene in Apples (Malus domestica Borkh. )
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摘要 通过设计引物进行PCR扩增、测序、拼接序列,以及与GenBank中的cDNA序列(登录号AY182241)进行比对,获得α-法尼烯合酶基因(AFS)的基因组序列和内含子/外显子结构。获得的AFS基因序列已在GenBank注册(登录号DQ901739),它有6个内含子和7个外显子,编码一个大小为576个氨基酸的蛋白质。间隔外显子的6个内含子相位分别是0、1、2、2、0、0,其大小分别是108、113、〉1000、125、220和88bp,7个外显子的推导氨基酸序列大小分别是57、89、127、73、48、83和99。编码的蛋白质中有3个序列模式(motif),RR(X8)W模式的编码基因序列在基因5′端的外显子1上,RxR模式和DDxxD模式的编码基因序列在外显子4上。将获得的AFS基因序列与GenBank中的cDNA序列(登录号AY523409)进行比对,结果发现两序列间有6个单核苷酸多态性,其中4个引起氨基酸突变。有意义的是,1个氨基酸突变(291R→G)发生在RxR模式上,此氨基酸突变以及其它突变是否与苹果α-法尼烯合成能力和虎皮病发生能力有关,值得进一步探讨。 Primer pairs were designed to amplify the genomic DNA of alpha-farnesene synthase gene (AFS) by PCR, the PCR products were sequenced, the sequences were spliced and compared to eDNA (accession No. AY182241 ) in the GenBank, then the genomic sequence and intron-exon organization of AFS gene were obtained. The AFS genomic sequence has been registered in GenBank (accession No. DQ901739), it had 6 introns and 7 exons, encoded a protein with 576 amino acids. The sizes of 6 introns were 108 bp, 113 bp, 〉1 1300 bp, 125 bp, 220 bp and 88 bp, and their phase were 0, 1, 2, 2, 0, 0, respectively. The sizes of deduced amino acids of 7 exons were 57, 89, 127, 73, 48, 83 and 99, respectively. The AFS protein contained three motifs, the RR ( X8 ) W motif was encoded by a sequence in exon 1, the RxR motif and DDxxD motif were encoded by two sequences in exon 4. The AFS genomic sequence ( accession No. DQ901739) was compared to eDNA ( accession No. AY523409) in the GenBank, it was found that there were 6 single-nucleotide polymorphisms between the two sequences, four of which caused mutations at the amino acid level. Interestingly, one amino acid mutation (291R→G) was found in RxR motif, it deserves further investigation whether the alpha-farnesene synthesis ability and superficial scald susceptibility of apples were influenced by this amino acid mutation and other mutations.
作者 苑克俊 刘庆忠 李勃 张力思 YUAN Ke-jun , LIU Qing-zhong, LI Bo, and ZHANG Li-si ( Shandong Institute of Pomology, Tai'an, Shandong 271000, China)
出处 《园艺学报》 CAS CSCD 北大核心 2007年第4期 1003-1006,共4页 Acta Horticulturae Sinica
基金 教育部留学回国人员科研启动基金资助项目[2004(527)] 山东省人事厅留学回国人员科技活动择优资助项目[2003(10)14]
关键词 苹果 α-法尼烯合酶 基因组DNA 多态性 虎皮病 Apple Alpha-farnesene synthase Genomic DNA Polymorphism Superficial scald
作者简介 E-mail: yuankj@vip. sina. com
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参考文献12

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