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A Primary Study of the Subgroups of T Lymphocytes in MHV-3 Induced Chronic Viral Hepatitis 预览 被引量:1

A Primary Study of the Subgroups of T Lymphocytes in MHV-3 Induced Chronic Viral Hepatitis
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摘要 为了在鼠科的肝炎病毒类型 3 的致病(MHV-3 ) 学习 T 房间子集的贡献,在 C3H/Hej 老鼠导致了长期的病毒的肝炎, 90 只 C3H/Hej 老鼠被选择个别地 intraperitoneally 收到 MHV-3 的 10 空斑形成单位(PFU ) 。病毒滴定率的变化;在肝织物的病理被标准匾试金检验;由 hematoxylin/eosin (他) 从 2 天柱子 MHV-3 感染染色方法。包括 CD3 的 T 房间子集的比率[+] CD4 [+] CD8 , CD3 [+] CD4 CD8 [+], CD3 [+] CD4 CD8 , CD3 [+] CD4 [+] CD25 [+], CD3 [+] CD4 [+] CD25 ;CD3 [+] CD4 CD25 [+] 在血的全部的 T 的 T,怒气;肝在 0 点被检验, 2, 4, 6, 8, 10, 12, 15, 20, 25, 30,由流动 cytosorting 的 40 天柱子 MHV-3 感染。我们观察到病毒滴定率涨了;显示出的持续性病毒复制;在从 2 天的 C3H/Hej 老鼠的肝的煽动性的变化张贴 MHV-3 感染。双否定 T 房间(DN Treg 房间) ;CD4 [+] CD25 [+] 从 2 天显著地增加的 T 房间比率在 C3H/Hej 鼠标张贴 MHV-3 感染,;CD3 [+] CD4 [+] CD8, CD3 [+] CD4 CD8 [+] , CD3 [+] CD4 [+] CD25;CD3 [+] CD4 CD25 [+] T 房间比率因此减少了。在结论,病毒滴定率的变化;在 C3H/Hej 老鼠柱子 MHV-3 的肝的病理建议他们的贡献到病毒的坚持。DN Treg 房间的进一步的描述是感染显示 MHV-3 能在 C3H/Hej 老鼠的肝导致长期的发炎。DN Treg 房间的增加;CD4 [+] CD25 [+] 在 C3H/Hej 鼠标的 T 房间比率张贴 MHV-3 感染建议那 DN Treg 房间;CD4 [+] CD25 [+] T 房间可以两个都在长期的病毒的肝炎的发展有重要镇压免疫调整功能;在病毒 persistent 感染有重要角色。推进 DNT 房间的描述;CD4 [+] CD25 [+] T 房间在调查下面。 To study the contribution of T cell subsets in the pathogenesis of Murine hepatitis virus Type 3(MHV-3) induced chronic viral hepatitis in C3H/Hej mice,ninety C3H/Hej mice were chosen to individually receive 10 plaque forming units(PFU)of MHV-3 intraperitoneally.The changes of virus titer and pathology in liver tissue were examined by standard plaque assay and by the hematoxylin/eosin(HE) staining method from 2 days post MHV-3 infection.The ratios of T cell subsets including CD3^+CD4^+CD8^-,CD3^+CD4^-CD8^+,CD3^+CD4^-CD8^-,CD3^+CD4^+CD25^+,CD3^+CD4^+CD25^-and CD3^+ CD4^-CD25^+ T lymphocyte of total T lymphocytes in blood,spleen and liver were examined at 0,2,4,6,8,10,12,15,20,25,30,40 days post MHV-3 infection by flow cytosorting.We observed that the virus titer raised and showed persistent virus duplications and inflammatory changes in the livers of C3H/Hej mice from 2 days post MHV-3 infection.The double negative T cell(DN Treg cell) and CD4^+CD25^+ T cell ratios increased significantly from 2 days post MHV-3 infection in C3H/Hej mice,and CD3^+CD4^+CD8^-,CD3^+CD4^-CD8^+,CD3^+CD4^+CD25^-and CD3^+CD4^-CD25^+ T cell ratios decreased accordingly.In conclusion,the changes of virus titer and pathology in the livers of C3H/Hej mice post MHV-3 suggest their contribution to viral persistence.Further characterizations of DN Treg cells are that infection indicates that MHV-3 could induce the chronic inflammation in livers of C3H/Hej mice.The increase of the DN Treg cell and CD4^+CD25^+ T cell ratios in C3H/Hej mice post MHV-3 infection suggests that DN Treg cells and CD4^+CD25^+ T cells may both have important suppressive immunomodulation functions in the development of chronic viral hepatitis and have important roles in the virus persistent infection.Further characterizations of DNT cell and CD4^+CD25^+ T cell are under investigation.
作者 Jiang-guo ZHANG Xiao-min QIN Xiao-jing WANG Wei-ming YAN Chuan-long ZHU Xiao-ping LUO Qin NING Jiang-guo Zhang[1]; Xiao-min Qin[1]; Xiao-jing Wang[1]; Wei-ming Yan[1]; Chuan-long Zhu[1]; Xiao-ping Luo[1] ; Qin Ning[1 ]&Email:qning@tjh.tjmu.edu.cn
出处 《中国病毒学》 CSCD 2007年第5期 339-346,共8页 Virologica Sinica
基金 Foundation items: National Nature Science Foundation (30571643, 30672380), Major State Basic Research Development Program of China (2005CB522901, 2007CB512904)
关键词 T淋巴细胞 亚群 慢性病毒性肝炎 MHV-3 MHV-3 Virus titer C3H/Hej Hematoxylin/eosin (HE) staining Flow cytosorting DNT lymphocyte
作者简介 Corresponding author. Tel: +86-27-83662391, E-mail: qning@tjh.tjmu.edu.cn
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