To study the influence of greenhouse cultivation on agriculture soil environment, the samples of soils and plants were collected from open fields and greenhouses in Hebei Province and were examined in three aspects of microbes, heavy metals and organophosphorus pesticides. Amounts of microorganism and structure of microbial community were examined with the method of diluted plate counting, and the microbial biomass of carbon was determined with the chloroform-fumigation and incubation method. Contents of heavy metals and residues of organophosphorus pesticides in soils and plants were also examined by atomic absorption spectrophotometry and GS-MS, respectively. The results showed a notable increase of bacteria and actinomycetes and a significant decrease of fungi in greenhouses, that is, the ratio of bacteria to fungi increased, which meant that the structure of the microbial community was still in a good situation. Meanwhile, the microbial biomass reduced along with the usage of greenhouses, which might indirectly reflect the increase of nutrients pressure. Accumulation of heavy metals and organophosphorus pesticides were not found in the greenhouses. Therefore, the study considered that short-term （20 years） intensive agricultural cultivation in greenhouses might not cause serious impact on the soil environment. The microbes in the agriculture soil system showed a sensitive response to the change of soil quality, which would be used as a good indicator of soil environment assessment.
Journal of Agro-Environment Science( J. Agro-Environ. Sci.)