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甘肃省天水及陇南部分地区虫媒病毒调查 预览 被引量:27

Investigation on arboviruses in Tianshui and Longnan regions of Gansu province
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摘要 目的对甘肃省天水陇南部分地区的吸血蚊虫进行病毒分离与鉴定。方法2006年8月在当地采集蚊虫标本,分离病毒和对病毒分离物进行血清学和分子生物学鉴定,以软件进行病毒的核苷酸序列比对和系统发生分析。结果分离到19株病毒。鉴定结果显示分离株GS10—2为盖塔病毒,GS42-2为版纳病毒。其余分离株为一种基因组约8000核苷酸的未知RNA病毒。盖塔病毒分离株3’UTR中具有与已往中国分离株相同的缺失序列和3个特异核苷酸位点。版纳病毒分离株基因组第12片段的进化关系同其它中国分离株有明显差异.位于一条独立的进化枝中。结论在该地区分离到1株盖塔病毒、1株版纳病毒和17株未知RNA病毒;盖塔病毒与以往我国分离株的遗传关系密切,版纳病毒与我国其它地区分离株有明显差异,在进化上相对独立。 In 2006,the arboviruses were isolated from mosquito specimens collected in Tianshui and Longnan regions of Gansu province by inoculating onto C6/36 and BHK-21 cells,and they were identified by serological and molecular biological methods. Sequence alignment and phylogenetic analysis on the viral isolates were carried out using Clustal X 1.8, GENEDOC and MEGA- 3.1 softwares. It was found that 19 strains of viruses were isolated from mosquitoes,among which one Getah virus (strain GS10-2),one Banna virus(strain GS42-2)and 17 strains of the unknown dsRNA viruses with about 8000 nucleotides in genome were isolated. As demonstrated by sequence analysis,the 3'UTR sequence of the Getah virus strain GS10-2 showed the same deletion as in the other viral isolates previously isolated in China and 3 specific nucleotide loci were found. The phylogenetic relationship of the 12 th segment in genome of the Banna virus isolate with other strains isolated in China demonstrated significant difference. From these observations,it is evident that the strain GS10-2 shows closely genetic relationship with other Getah virus isolates in China, but strain GS42-2 has a distinct phylogenetic relationship with the Banna virus strains isolated from other regions of China.
作者 翟友刚 王焕琴 许海魁 孟维珊 曹玉玺 付士红 梁国栋 ZHAI You-gang,WANG Huan-qing, XU Hai-kui,MENG Wei-shan,CHAO Yu- xi,FU Shi- hong, LIANG Guo-dong (State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, China CDC , Beijing 100052, China)
出处 《中国人兽共患病学报》 CAS 北大核心 2008年第2期 95-99,共5页 Chinese Journal of Zoonoses
基金 科技部重大传染病专项(2003BA712A08-01)
关键词 虫媒病毒 盖塔病毒 版纳病毒 序列分析 系统发生 arbovirus getah virus banna virus sequence analysis phylogeny
作者简介 通讯作者:梁国栋,Email:gdliang@hotmail.com
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