BACKGROUND： Previous research reveals that, the morphous, structure, physicochemical property and mechanical characteristics of matrix in buffalo cornu all contribute to the biomaterials in the field of orthopaedics.
OBJECTIVE： To investigate the correlation between the duration of matrix in buffalo cornu soaked in 30% hydrogen peroxide and the antigenicity, the biomechanical intensity of matrix in buffalo cornu treated by deproteinization.
DESIGN, TIME AND SETTING： Measurement experiments were performed in the Mechanics Laboratory, Kunming University of Science and Technology （Kunming, Yunnan, China） from February to March in 2000.
MATERIALS： Buffalo was provided by Luxi Abattoir of Yunnan Province （China）, and then processed into test pieces at a nominal thickness.
METHODS： The metric test pieces were rinsed with distilled water and soaked by chloroform-methyl alcohol at a ratio of 1：1 for 48 hours, then in 30% hydrogen peroxide for 24 and 48 hours, which served as the experimental group. While those remaining untreated were taken as controls.
MAIN OUTCOME MEASURES： The modulus of elasticity and compressive strength were measured by electric measurement method and direct pressurization method, respectively.
RESULTS： The modulus of elasticity between the experimental group and the control group had no significant difference （P 〉 0.05）. The compressive strength in the control group was significantly higher than that in the experimental group （P 〈 0.01）, and there were no significant differences between the experimental group treated for 24 hours and the experimental group treated for 48 hours （P 〉 0.05）.
CONCLUSION： Subsequent to soaking in 30% hydrogen peroxide for 48 hours, the matrix in buffalo cornu maintains better biomechanical intensity.
Journal of Clinical Rehabilitative Tissue Engineering Research
Acknowledgement Thanks for Wen Hong-guang and Luo Cheng-ling et al in the Kunming University of Science and Technology with regard to the mechanical experiments.