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TEG技术在血小板保存过程中功能评价的应用研究 预览 被引量:9

Application of Thrombelastography in Evaluation of Platelet Function during Storage
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摘要 本研究旨在通过血栓弹力图(thmmbelastography,TEG)技术探讨血小板保存过程中功能的变化。随机选择各项指标均符合国家标准的单供者机采血小板12个单位并在(22±2)℃条件下振荡保存。分别在保存1、2、3、4、5天检测血栓弹力图参数,包括反应时间(R)、凝血时间(K)、α角(ANG)和最大振幅(MA),同时检测血小板计数、平均血小板体积、低渗休克反应(HSR)水平、CD62p阳性率及凝血酶激活CD62p再表达率的变化,综合评价血小板保存过程中体外功能的变化情况。结果显示:平均血小板体积随保存时间延长而轻度增大,但无统计学差异(p〉0.05);血小板膜表面CD62p表达率随保存时间延长而显著升高(P〈0.01);凝血酶激活后CD62p再表达率随保存时间的延长而显著下降(p〈0.01);血小板低渗休克反应水平在1-5天无明显变化(P〉0.05);R值随保存时间延长而明显延长(P〈0.01),K值无明显变化(P〉0.05),α角虽呈轻度下降趋势,但无显著差异(P〉0.05);MA值在保存1-4天无显著变化,保存5天时仅有轻度下降(P〈0.05)。结论:虽然血小板随保存时间延长激活率明显升高,但反映血小板综合凝血功能的最大振幅(MA值)和HSR水平在整个保存期内并无显著变化,说明保存期末的血小板仍然具有良好的止血功能;血栓弹力图参数MA值可以作为一项重要指标用于血小板保存过程中的功能评价。 This study was aimed to explore changes of platelet function in vitro during storage by thrombelastography (TEG). 12 units plateletpheresis were randomly selected and stored at 20 to 24℃ with agitation. Thrombelastography variable parameters R,K values and maximal amplitude (MA) were measured on 1,2,3,4,5 days of platelet storage. Platelet concentration, mean platelet volume( MPV), hypotonic shock response (HSR) , CD62p expression and CD62p reexpression on platelet surface were detected at the same time. Changes of platelet function in virto were systematically evaluated by above-mentioned indexes. The results showed that MPV augmented slightly with prolongation of preserved time(p 〉 0.05 ), and CD62p expression on platelet surface increased remarkably(p 〈 0.01 ), while CD62p reexpression decreased gradually(p 〈 0.01 ). There were no significant differences in HSR level of platelets during storage (p 〉 0. 05). R value increased with prolongation of preserved time(p 〈 0.01 ). There were no obvious changes on K value and αAngle during storage (p〉0.05). There were no obvious changes in MA from 1 to 4 days, and MA decreased slightly on day 5 (p 〈 0.05 ). It is concluded that there was no significant change in MA and HSR which reflects comprehensive coagulation of platelets during storage. Platelets on the end of storage have excellent function of hemostasis; Thrombelastography parameter MA value can be used as a valuable indicator for evaluation of platelet function in vitro during storage.
作者 于洋 林子林 冯倩 潘纪春 张婷 孙桂香 张小娟 马春娅 葛国峰 汪德清 骆群 田亚平 YU Yang, LIN Zi-Lin, FEN Qian, PAN Ji-Chun, ZHANG Ting, SUN Gui-Xiang, ZHANG Xiao-Juan, MA Chun-Ya, GE Guo-Feng, WANG De-Qing, LUO Qun, TIAN Ya-Ping (Department of Blood Transfusion, Center for Clinical Transfusion Medicine, PLA General Hospital, Beijing 100853, China)
出处 《中国实验血液学杂志》 CAS 2008年第4期 926-929,共4页 Journal of Experimental Hematology
关键词 血栓弹力图 血小板保存 低渗休克反应 CD62P thrombelastography platelet storage hypotonic shock response CD62p
作者简介 通讯作者:田亚平,主任医师、教授、博士生导师.电话:(010)66939374.E—mail:tianyp@301hospital.com.cn
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