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白姜花倍半萜合成酶基因的克隆及表达 预览 被引量:4

Molecular Cloning and Expression of Sesquiterpenoid Synthase Gene in Hedychium coronarium Koenig
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摘要 以白姜花的叶片为材料,通过RT—PCR与RACE相结合的方法,克隆到一个倍半萜合成酶基因的cDNA序列,其全长为1932bp,基因编码区共1653bp,编码551个氨基酸,命名为Hc—Sesqui。该基因编码蛋白的氨基酸序列与姜和玉米中的倍半萜合成酶有较高的同源性,并且含有DDXXD保守序列。通过ClustalX进行序列分析,确定该基因属于植物萜类合成酶基因家族中的Tps-a亚族。Northern杂交的结果表明,该基因在茎、叶和萼片中均有表达。 A TPS gene named as Hc-Sesqui was isolated from Hedychium coronarium Koenig leaf. The complete sequence of the cDNA gene was 1 932 bp, with an ORF encoding 551 amino acids. The amino acids sequence shared highly homology to other sesquiterpenoid synthase, containing conserved boxes of: TPS DDXXD. The phylogenetic analysis after Clustal X alignment suggested that the Hc-Sesqui belonged to Tps-a. Northern blot revealed that the Hc-Sesqui gene was expressed in leaf, stem and sepal.
作者 李瑞红 范燕萍 余让才 陆旺金 庄楚雄 LI Rui-hong, FAN Yan-ping , YU Rang-cai , LU Wang-jin , ZHUANG Chu-xiong (1. College of Horticulture; 2 College of Life Science, South China Agricultural University, Guangzhou 510642, China)
出处 《园艺学报》 CAS CSCD 北大核心 2008年第10期 1527-1532,共6页 Acta Horticulturae Sinica
基金 广东省自然科学基金项目(07006667) 广东省科技计划项目(20078050200020)
关键词 白姜花 倍半萜合成酶 基因 克隆 表达 Hedychium coronarium Koenig sesquiterpenoid synthase gene clone expression
作者简介 通讯作者Author for correspondence(E-mail:fanyanping@scau.edu.cn)
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参考文献13

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