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丙型肝炎病毒基因1b型F蛋白对人肝细胞癌细胞株HepG2凋亡的影响 被引量:1

Influence of F protein of hepatitis C virus subtype 1b inhibits on human hepatocellular carcinom HepG2 cell apoptosis
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摘要 目的研究丙型肝炎病毒(HCV)基因1b型F蛋白对人肝细胞癌细胞株HepG2凋亡的影响。方法构建真核表达重组质粒pcDNA3.0-F—EGFP,并利用Lipofectamine2000转染人肝细胞癌细胞系HepG2,同时设pcDNA3.0-C-EGFP-HepG2阳性对照、pcDNA3.0-HepG2为阴性对照及未转染HepG2为空白对照,以Act-D、TNFα诱导四组细胞系发生凋亡,Annexin V-FITC/PI双染检测肿瘤细胞凋广率,并使用细胞凋亡DNA Ladder检测,进一步验证HCV 1b型F蛋白在肝细胞凋亡的生物学功能效应。结果pcDNA3.0-F-EGFP-HepG2细胞系在发生凋亡的时间上比pcDNA3.0-C-EGFP-HepG2快,但相对空白质粒转染组及未转染HepG2细胞系有明显延迟,凋亡的发生率也明显低于阴性对照及空白对照细胞系(P〈0.001)。结论外源性基因HCV1b型F的引入及表达,对HepG2细胞的凋亡有抑制作用。 Objective To investigate the effects of F protein of hepatitis C virus subtype lb on the apoptosis of human hepatocellular carcinom HepG2 cells. Methods HepG2 cells were transfected with recombinant plasmid pcDNA3.0-F-EGFP and pcDNA3.0-F-EGFP-HepG2 strain was exposed to Act-D and tumor necrosis factor α (TNFα ) treatment in order to induce cell apoptosis with positive control pcDNA3.0-C-EGFP-HepG2, negative control pcDNA3.0-C-EGFP-HepG2 and blank control HepG2. Annexin V-FITC/PI of flow cytometry was performed to determine the number of apoptotic cells. DNA Ladder was used to observe the isolation of apoptotic DNA fragments in the apoptotic cells. Results pcDNA3.0-F-EGFP- HepG2 cell strain showed a much delayed apoptosis as well as obviously lowering the apoptotic rate when compared with the pcDNA3.0-HepG2 strain and HepG2 strain (P〈0.001). Conclusion The introduction and expression of extraneous gene (the F gene of hepatitis C virus subtype 1 b) could significantly inhibit the apoptosis of HepG2 cells.
作者 杨静静 张云 邓小昭 许可 王忠灿 王洁 冯乐 丁伟良 YANG Jing-jing, ZHANG Yun, DENG Xiao-zhao, XU Ke, WANG Zhong-can, WANG Jie, FENG Le, DING Wei-liang. (School of Public Health, Nanjing Medical University, Nanjing 210029, China)
出处 《中华流行病学杂志》 CAS CSCD 北大核心 2009年第4期388-392,共5页 Chinese Journal of Epidemiology
基金 基金项目:江苏省高校自然科学基金(03KJD330144)
关键词 丙型肝炎病毒 F蛋白 凋亡 肿瘤坏死因子Α Hepatitis C virus F protein Apoptosis Tumor necrosis factor α
作者简介 通信作者:张云,Email:zhangyun111@sohu.com
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参考文献14

  • 1Leone N, Rizzetto M. Natural history of hepatitis C virus infection: from chronic hepatitis to cirrhosis, to hepatocellular carcinoma. J Minerva Gastroenterol Dietol, 2005, 51 ( 1 ) : 31-46. 被引量:1
  • 2丙型肝炎防治指南[J].中华传染病杂志,2004,22(2):131-136. 被引量:215
  • 3Chang J, Yang SH, Cho YG, et al. epatitis C virus core from two different genotypes has an oncogenic potential but is not sufficient for transforming primary rat embryo fibroblasts in cooperation with the H-ras oncogene. J Virol, 1998, 72 (4) : 3060-3065. 被引量:1
  • 4Kwun H J, Jung EY, Ahn JY, et al. p53-dependent transcriptional repression of p21 (wafl) by hepatitis C virus NS3. J Gen Virol, 2001,82(Pt 9) :2235-2241. 被引量:1
  • 5Gong G, Wafts G, Tanveer R, et al. Human hepatitis C virus NS5A protein alters intracellular calcium levels, induces oxidative stress, and activates STAT-3 and NF-kappa B. Proc Natl Acad Sci USA, 2001,98 (17) : 9599-9604. 被引量:1
  • 6Ogata S, Nagano-Fujii M, Ku Y, et al. Comparative sequence analysis of the core protein and its fi-ameshift product, the F protein, of hepatitis C virus subtype lb strains obtained from patient with and without hepaocellular carcinoma. J Clin Microbiol, 2002,40 (10) : 3625-3630. 被引量:1
  • 7Jones BE, Lo CR,Liu H, et al. Hepatocytes sensitized to tumor necrosis factor: a cytotoxicity undergo apoptosis through caspasedependent and caspase-independent pathways. J Biol Chem, 2000, 275( 1 ) : 705-712. 被引量:1
  • 8Xu Z, Choi J, Yen TS, et al. Synthesis of a novel hepatitis C virus protein by ribosomal frameshift. EMBO J, 2001,20 (14) : 3840-3848. 被引量:1
  • 9Walewski JL, Keller TR, Stump DD, et al. Evidence for a new hepatitis C virus antigen encoded in an overlapping reading frame. RNA, 2001,7:710-721. 被引量:1
  • 10Boulant S, Becchi M, Penin F, et al. Unusual multiple recoding events leading to alternative forms of hepatitis C virus core protein from genotype 1b. J Biol Chem, 2003,278 (46) : 45785- 45792. 被引量:1

二级参考文献13

  • 1丙型肝炎防治指南[J].临床肝胆病杂志,2004,20(4):197-203. 被引量:722
  • 2Rosen HR,Gretch DR.Heptitis C virus:current understanding and prospects for future therapies.Mol Med Today,1999,5:393-399. 被引量:1
  • 3Xu Z,Choi J,Yen TS,et al.Synthesis of a novel hepatitis C virus protein by ribosomal frameshift.EMBO J,2001,20:3840-3848. 被引量:1
  • 4Varaklioti A,Vassilaki N,Georgopoulou U,et al.Alternate translation occurs within the core coding region of the hepatitis C viral genome.J Biol Chem,2002,277:17713-17721. 被引量:1
  • 5Boulant S,Becchi M,Penin F,et al.Unusual multiple recoding events leading to alternative forms of hepatitis C virus core protein from genotype 1b.J Biol Chem,2003,278:45785-45792. 被引量:1
  • 6Lohmann V,Komer F,Koch J,et al.Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line.Science,1999,285:110-113. 被引量:1
  • 7Walewski JL,Keller TR,Stump DD,et al.Evidence for a new hepatitis C virus antigen encoded in an overlapping reading frame.RNA,2001,7:710-721. 被引量:1
  • 8Bain C,Parroche P,Lavergne JP,et al.Memory T-cell-mediated immune responses specific to an alternative core protein in hepatitis C virus infection.J Virol,2004,78:10460-10469. 被引量:1
  • 9Komurian-Pradel F,Rajoharison A,Berland JL,et al.Antigenic relevance of F protein in chronic hepatitis C virus infection.Hepatology,2004,40:900-909. 被引量:1
  • 10康来仪,孙永德.我国丙型和戊型肝炎人群流行病学调查及流行因素的研究[J].中华传染病杂志,1997,15(2):71-75. 被引量:97

共引文献222

同被引文献13

  • 1蒋春梅,刁振宇,崔国兴,钱万红,邓小昭,许可,丁伟良,谈永飞,张云.丙型肝炎病毒F蛋白的原核表达及初步应用[J].细胞与分子免疫学杂志,2007,23(2):134-137. 被引量:7
  • 2Leone N, Rizzetto M. Natural history of hepatitis C virus infection: from chronic hepatitis to cirrhosis, to hepatocellular carcinoma[ J]. J Minerva Gastroenterol Dietol, 2005, 51 ( 1 ) : 31 - 35. 被引量:1
  • 3Basu A, Steele R, Ray R, et al. Functional properties of a 16 kDa protein translated from an alternative open reading frame of the coreencoding genornic region of hepatitis C virns[J]. J Gen Virol, 2004, 85 (8) : 2299 - 2306. 被引量:1
  • 4Roussel J, Pillez A, Montpellifr C, et al. Characterization of the expression of the hepatitis C virus F protein[J]. J Gen Virol, 2003, 84 (Pt 7) : 1751 - 1759. 被引量:1
  • 5Boulant S, Becchi M, Penin F, et al. Unusual multiple recod lng events leading to alternative forms of hepatitis C virus core protein from genotype lb[J]. J Biol Chem, 2003, 278(46) : 45785 -45792. 被引量:1
  • 6Ruggieri A, Murdolo M, Harada T, et al. Cell cycle perturbation in a human hepatoblastoma cell line constitutively expressing hepatitis C virus core protein[ J]. Arch Virol, 2004, 149( 1 ) : 61 -74. 被引量:1
  • 7Watashi K, Hijikata M, Marusawa H, et al. Cytoplasmic localization is important for transcription factor nuclear factor-kB activation by hepatitis C virus core protein through its amino terminal region [ J ]. Viralogy, 2001, 286(2): 391 -402. 被引量:1
  • 8Branch AD, Stump DD, Gutierrez JA, et al. The hepatitis C virus alternate reading frame (ARF) and its family of novel products: the alternate reading frame protein/F-protein, the double-frameshift protein, and others[ J]. Semin Liver Dis, 2005, 25 ( 1 ) : 105 - 117. 被引量:1
  • 9宋其蔓(综述),孙洞箫(审校).细胞凋亡与p21关系的研究进展[J].医学综述,2008,14(6):816-818. 被引量:18
  • 10钟馨,喻陆.松花粉对人胚肺二倍体成纤维细胞p16及p21基因表达的影响[J].浙江中医药大学学报,2008,32(1):89-92. 被引量:1

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