期刊文献+

水分胁迫诱导八棱海棠和平邑甜茶细胞程序性死亡的研究 被引量:11

Research of Programmed Cell Death Under Water Stress in Malus robusta Rehd. and Malus hupehensis (Pamp.) Rehd.
分享 导出
摘要 以苹果属抗旱能力较强的八棱海棠(Malus robusta Rehd.)和较弱的平邑甜茶[Malus hupehensis(Pamp.)Rehd.]水培幼苗为试验材料,利用细胞形态学、DNAladder等技术,用20%PEG6000模拟水分胁迫进行诱导细胞程序性死亡研究。经DAPI染色后荧光显微镜观察发现两个苹果属材料均呈现细胞染色质凝集、细胞核缩小、变形、解体等细胞程序性死亡的形态学特征;同时,DNA琼脂糖凝胶电泳可观察到明显的“梯状”DNA条带(DNA ladder)。由此表明,水分胁迫诱导苹果属植物具有明显的细胞程序性死亡的典型形态及生化特征,是一种主动的细胞死亡过程,八棱海棠细胞程序性死亡持续的时间长于平邑甜茶。 The seedling of Malus robusta Rehd. and Malus hupehensis ( Pamp. ) Rehd. were treated with 20% PEG 6000. The study was to observe programmed cell death under water stress through morphocytology and DNA ladder. The nuclear morphology was observed after DAPI staining. And the results showed that the characteristic morphological changes of chromatin/chromosome and nuclear such as chromatin condensed, karyon shinked, distorted and degraduated were found with stress time. Meantime, DNA ladder was found in cells after agarose gel electrophoresis. Cell death induced by water stress showed typical morphological and bi- ochemical properties of programmed cell death, and this was an active cell death. Additionally, PCD in Malus robusta Rehd. was longer than in Malus hupehensis (Pamp.) Rehd. seedlings.
作者 曹慧 李春霞 王孝威 邹岩梅 束怀瑞 CAO Hui, LI Chun-xia, WANG Xiao-wei,ZOU Yan-mei, and SHU Huai-rui (1.Institute of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China; 2 Weifang University, Weifang , Shandong 261061, China; 3 Shanxi Agricultural University, Taigu , Shanxi 030801, China; 4Yanan Vocational and Technical College, Yanan, Shaanxi 716000, China)
出处 《园艺学报》 CAS CSCD 北大核心 2009年第4期469-474,共6页 Acta Horticulturae Sinica
基金 山东省中青年科学家奖励基金项目(2005BS02012) 山西省自然科学基金项目(20051068)
关键词 苹果属 水分胁迫 程序性死亡 DAPI DNA LADDER Malus water stress programmed cell death DAPI DNA ladder
作者简介 通讯作者Author for correspondence(E-mail:hrshu@sdau.edu.cn)
  • 相关文献

参考文献19

  • 1Danon A, Gallois P. 1998. UV-C induces apoptotic-like changes in Arabidopsis thaliana. FEBS Lett, 437:131 -136. 被引量:1
  • 2Gunawardena A H L, Pearce D M, Jackson M B, Hawes C R, Evans D E. 2001. Characterization of programmed cell death during aerenchyma formation induced by ethylene or hypoxia in roots of maize (Zea mays L. ). Planta, 212:205 -214. 被引量:1
  • 3郭晓军,田国忠,赵少波,李树卿.抗枣疯病枣树品种的DAPI荧光染色检测[J].河北林业科技,2003(4):1-3. 被引量:7
  • 4纪睿,张正光,王源超,郑小波.疫霉菌激发子PB90诱导烟草悬浮细胞的凋亡[J].科学通报,2005,50(5):448-452. 被引量:3
  • 5Katsuhara M. 1997. Apoptosis-like cell death in barley roots under salt stress. Plant Cell Physiol, 38 : 1091 - 1093. 被引量:1
  • 6Koukalova B, Kovarik A, Fajkus J, Siroky J. 1997. Chromatin fragmentation associated with apoptotic changes in tobacco cells exposed to cold stress. FEBS Lett, 414 : 289 - 292. 被引量:1
  • 7Lam E, Katon N, Lawton M. 2003. Programmmed cell death, mitochondria and the plant hypersensitive response. Nature, 411 : 848 -853. 被引量:1
  • 8李荣峰,蔡妙珍,刘鹏,徐根娣,陈敏燕,梁和.Al^3+对大豆根边缘细胞程序性死亡诱导的生理生态作用[J].植物生态学报,2008,32(3):690-697. 被引量:10
  • 9Liljeroth E, Bryngelsson T. 2001. DNA fragmentation in cereal roots indicative of programmed root cortical cell death. Physiol Plant, 111 : 365 - 372. 被引量:1
  • 10马怀宇..水分和盐胁迫下平邑甜茶根系细胞程序性死亡研究 [D].山东农业大学,2006:

二级参考文献123

共引文献64

同被引文献187

引证文献11

二级引证文献23

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈