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α-硅酸钙和β-硅酸钙的体外体内降解性研究 预览 被引量:3

Comparative Study the Degradation of α-CaSiO3 and β-CaSiO3 Bioactive Bioceramics in Vivo and in Vitro
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摘要 研究α-CS和β-CS多孔硅酸钙生物陶瓷的体内、体外降解性能,并与β-磷酸三钙(β-TCP)多孔生物陶瓷比较。多孔生物陶瓷由添加造孔剂和高温烧结工艺制备得到。体外实验:将陶瓷样品在三羟甲基胺基甲烷-盐酸(Tris-HCl)缓冲溶液中浸泡1、3和7天后,通过测量材料的失重率来评价材料的降解性;体内实验:在新西兰大耳白兔股骨髁骨缺损处植入人工骨陶瓷,于术后第1周、4周、8周和12周收集标本,进行组织学观察并计算残余材料的面积百分比,分析材料的体内降解性能。体外降解性实验结果显示,三种材料的降解率β-CS〉α-CS〉β-TCP。β-CS和α-CS间无统计学差异(P〉0.05),各时间点两种材料均与β-TCP有统计学差异(P〈0.05)。体内降解性实验结果显示,三种材料随时间延长都表现出降解,降解率β-CS〉α-CS〉β-TCP,但是统计学结果显示没有差异(P〉0.05)。β-CS和α-CS多孔生物活性陶瓷的降解性均明显优于β-TCP多孔生物陶瓷。研究结果显示β-CS和α-CS多孔生物陶瓷均有望作为硬组织修复和骨组织工程支架材料使用。 The degradability of the α-CS and β-CS bioactive macroporous bioceramic in vitro and in vivo were comparatively studied, and the results were compared with β-tricalcium phosphate ( β-Ca3 ( PO4 )2, β-TCP) macroporous bioceramics. The macroporous α-CS, β-CS and β-TCP bioceramics were obtained after sintering at high temperature . The degradability of macroporous bioceramics in vitro was determined by their weight loss percentage in Tris - HCl buffer solution after soaking for 1, 3 and 7 days. In the experiment in vivo, the ceramic samples were implanted in a rabbit condyles of femur defect model, and the specimens were harvested after 1,4 , 8 and 12 weeks, respectively. Degradation of the grafts were evaluated by the means of histological and histomor- phometry after 1, 4 , 8 and 12 weeks of implantation. The experiment in vitro result showed that the degradation rate was β-CS 〉 α-CS 〉 β-TCP. There is no obviously difference in statistics between β-CS and α-CS ( P 〉 0.05 ). There is obviously difference in statistics between β-TCP and CS. In the experiment in vivo, the histological and histomorphometry results showed that the degradation rate was β-CS 〉 α-CS 〉 β-TCP, but there is no obviously difference in statistics. The present studies showed that the degradability of the porous β-CS and α-CS ceramics is much faster than that of β-TCP. Therefore, both of the β-CS and α-CS macroporous bioceramics could be used as bioactive and biodegradable materials for hard tissue repair and tissue engineering applications.
作者 刘勇 王臻 林开利 常江 刘鹏 LIU Yong, WANG Zhen , LIN Kai-li, CHANG Jiang ,LIU Peng (Institute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, P. R. China; Shanghai Institute of Ceramics, Chinese Academy of Sciences , Shanghai 200050, P. R. China)
出处 《科学技术与工程》 2009年第10期 2569-2573,2582,共6页 Science Technology and Engineering
基金 国家自然科学基金重点项目(30730034)、上海市科委项目(07ZR14127)资助
关键词 骨缺损 骨移植 骨再生 硅酸钙 磷酸三钙 人工骨 降解 bone defect bone transplantation bone regeneration calcium silicate tricalcium phosphate artificial bone degradiation
作者简介 刘勇(1981-),男,河北高邑人,汉族,硕士生,研究方向:骨组织工程。E-mial:dragonliu999@126.com。 通讯作者简介:王臻。E-mial:wangzhen@fmmu.edu.cn。
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