期刊文献+

猪瘟病毒Thiverval株3′非编码区插入片段对病毒拯救的影响 预览

Influence of insertion in the 3′ untranslated region on the rescue of attenuated classical swine fever virus
在线阅读 下载PDF
收藏 分享 导出
摘要 猪瘟病毒(CSFV)疫苗株Thiverval株与亲本病毒Alfort株相比,最明显的区别就是其3′-UTR含有一定长度的插入序列。本研究通过反向遗传学操作,构建了3′-UTR含有19nt、32nt以及插入片段完全缺失的CSFVThiverval株全长cDNA感染性克隆,将突变病毒基因组进行体外转录,利用BHK-21细胞转染、PK-15细胞传代增殖的方式成功的从3种重组突变感染性克隆中拯救出活病毒粒子,表明CSFV疫苗株3′-UTR插入片段并不影响病毒的拯救。通过3′-UTR二级结构模拟,发现疫苗株的插入片段导致3′-UTR空间构型发生改变、自由能升高、影响二级结构的稳定性,可能是导致疫苗株毒力减弱的原因之一。 The Thiverval vaccine strain of classical swine feuer virus (CSFV) differs from its parent Alfort strain by having an insertion in the 3′-UTR of genome. In this study, a series of CSFV Thiverval strain infectious cDNA clone with different insertions or complete deletion of insertion in the 3′-UTR were constructed through reverse genetics. Viruses were rescued from all the cDNA clone using the BHK-21 cells for transfection and PK-15 cells for multiplication. The results showed that insertion in 3′-UTR of attenuated CSFV did not effect the virus rescue, and it was not the decisive factor for maintaining viable virus. Secondary structure Modeling of the 3′-UTR suggests that the T-rich insertion could result in the change of structure and free energy, thus affecting the stability of the 3′-UTR structure, which could account for the attenuation of vaccine strain.
作者 范运峰 赵启祖 赵耘 邹兴启 张仲秋 王琴 宁宜宝 FAN Yun-feng, ZHAO Qi-zu, ZHAO Yun, ZOU Xing-qi, ZHANG Zhong-qiu, WANG Qin, NING Yi-bao (1. China Institute of Veterinary Drug Control, Beijing 100081, China 2. China Animal Disease Control Center, Beijing 100026, China)
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2009年第6期 420-423,共4页 Chinese Journal of Preventive Veterinary Medicine
基金 国家自然科学基金(30571377) 国家863计划(2006AA10A204)
关键词 猪瘟病毒 3′非编码区 插入片段 病毒拯救 二级结构分析 CSFV 3′-UTR insertion virus rescue RNA secondary structure
作者简介 范运峰(1980-),男,山西运城人,博士,主要从事分子病毒学与免疫学研究. 通信作者:E-mail:zhaoqizu@ivdc.gov.cn
  • 相关文献

参考文献8

  • 1Moennig V,Plagemann P G.The pestiviruses[J].Adv Virus Res,1992,41:53-98. 被引量:1
  • 2Behrens S E,Grassmann C W,Thiel H J,et al.Characterization of an autonomous subgenomic pestivirus RNA replicon[J].J Virol,1998,72:2364-2372. 被引量:1
  • 3Yu H,Grassmann C W,Behrens S E.Sequence and structural elements at the 3' terminus of bovine viral diarrhea virus genomic RNA:functional role during RNA replication[J].J Virol,1999,73:3638-3648. 被引量:1
  • 4Wu H X,Wang J F,Zhang C Y,et al.Attenuated lapinized chinese strain of classical swine fever virus:complete nucleotide sequence and character of 3'-noncoding region[J].Virus Genes,2001,23:69-76. 被引量:1
  • 5Vilcek S,Belak S.Organization and diversity of the 3'-noncoding region of classical swine fever virus genome[J].Virus Genes,1997,15:181-186. 被引量:1
  • 6Bjorklund H V,Stadejek T,Vilcek S,et al.Molecular characterization of the 3' noncoding region of classical swine fever virus vaccine strains[J].Virus Genes,1998,16:307-312. 被引量:1
  • 7Fan Y F,Zhao Q Z,Zhao Y,et al.Complete genome sequence of attenuated low-temperature Thiverval strain of classical swine fever virus[J].Virus Genes,2008,36(3):531-538. 被引量:1
  • 8Gong Y,Trowbridge R,Macnaughton T B,et al.Characterization of RNA synthesis during a one-step growth curve and of the replication mechanism of bovine viral diarrhoea virus[J].J Gen Virol,1996,77:2729-2736. 被引量:1
投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈
新型冠状病毒肺炎防控与诊疗专栏