目的研究细胞外信号调节激酶1/2（extracellular regulated kinase 1/2,ERK1/2）及其磷酸化状态（p-ERK1/2）在不同分化程度肺癌中的表达情况,探讨二者与肺癌侵袭、转移的关系。方法采用免疫组化（Envision）法,检测79例肺癌组织及l2例癌旁正常肺组织中ERK1/2和p-ERK1/2的表达。结果ERK1/2在高、中、低分化组表达率分别为13.6%,39.4%,66.7%,p-ERK1/2在高、中、低分化组表达率分别14.3%,27.3%,79.2%（P〈0.05）;无淋巴结转移者阳性率为20%,有淋巴结转移者阳性率为50.1%（P〈0.05）。ERK1/2和p-ERK1/2的表达在不同年龄、性别、肿瘤大小、肿瘤病理类型无显著性差异,而与分化程度有关,其中p-ERK1/2的表达还与有无淋巴结转移有关。结论ERK1/2和p-ERK1/2在肺癌组织中高表达且与分化程度有关。
Objective To detect the expression of ERK1/2 and p-ERK1/2 in different lung cancer and research the relationship between the two factors and the lung cancer. Methods The expression of ERK1/2 and p-ERK1/2 in different human lung cancers were detected by immunohistochemistry techniques. Results The expression of ERK in the poorly, moderate and well differentiated groups was 13.6%, 39.4% and 66.7%, respectively, while the expression of p-ERK1/2 was 14. 3%, 27. 3% and 79.2% （P〈0. 05）. The positive rate was 51.3% in cases with lymph node metastasis and 20% in those without. The expressions of ERK1/2 and p-ERK1/2 proteins were related with differentiation degree of lung cancer, but not with sex, age, pathological type and site or size of tumor; and the expression of p- ERK1/2 was also related with Lymph node metastasis. Conclusion ERK1/2 and p-ERK1/2 protein expressed more in different human lung cancer, they may play important roles in the occurrence and development of lung cancer.
Chinese Journal of Histochemistry and Cytochemistry
谷艳娇，女（1970年），汉族，硕士研究生。通讯作者（To whom correspondence should be addressed）