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金荞麦提了物抑制肿瘤细胞侵袭,转移和HT—1080细胞产生IV型胶原酶 … 预览 被引量:48

Extract of fagopyrun cymosum meissn inhibits tumor cell invasion and metastasis and suppresses type Ⅳ collagenase production of HT1080 cells
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摘要 目的 探讨金荞麦提取物对肿瘤细胞侵袭和转移的影响。方法 以人工重组基底膜及小鼠黑色素瘤转移株自发性肺转移模型观察了金荞麦提取的对B16-BL6细胞的体外抗侵袭活性和体内抗转移作用。用聚丙烯酰胺凝胶电泳地进一步观察了其对人纤维肉瘤HT-1080细胞IV型胶原酶的产生及活性的影响。同时用WST法观察了该药的细胞毒性。结果 金荞麦提取物在100mg.L^-1剂量下能有效抑制B16-BL黑色素瘤细胞在C5 AIM To study antiinvasive and antimetastatic activities of fagopyrun cymosum meissn on B16BL6 melanoma cells (highly metastatic cell line) and inhibitory effect on type Ⅳ collagenase production of HT1080 cells. METHODS Reconstituted basement membrane invasion assay was utilized to evaluate the invasive activity of cancer cells. Tyep Ⅳ collagenase production was assessed by PAGE substrate zymography. WST assay was used to examine the cytotoxicity of fagopyrun cymosum meissn. RESULTS The extract of fagopyrun cymosum meissn significantly inhibited membrane invasion of B16BL6 bearing C57/BL6 cell at a concentration of 100 mg·L-1 and effectively suppressed the spontaneous lung metastasis on B16BL6 bearing C57/BL6 mice at the dosage of 200 mg·kg-1. Extract of fagopyrun cymosum inhibited the production of type Ⅳ collagenases of human fibrosarcoma cells line HT1080 but it has no effect on the activity of this enzyme. CONCLUSION Extract of fagopyrun cymosum meissn has the notable inhibitory effects on tumor invasion and metastasis. Type Ⅳ collagenase inhibition may correlate with the antiinvasive and antimetastatic activities of Fagopyrun cymosum.
作者 刘红岩 韩锐 LIU HongYan, HAN Rui(Institute of Materia Medica, Peking Union Medical Collegeand Chinese Academy of Medical Sciences, Beijing 100050)
出处 《中国药理学通报》 CAS CSCD 北大核心 1998年第1期 36-39,共4页 Chinese Pharmacological Bulletin
基金 日本文部省富山医科药科大学和汉药研究所资金资助
关键词 金荞麦 提取物 肿瘤 转移 抑制作用 IV型胶原酶 fagopyrun cymosum meissn invasion metastasis type Ⅳ collagenases
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