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中子辐射诱发人外周血染色体畸变、微核HPRT基因突变的比较研究 被引量:1

Comparative Study on Human Peripheral Blood Chromosome Aberration,Micronucleus and HPRT Gene Mutation Induced by Neutron Irradiation
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摘要 目的研究中子辐射诱发离体人外周血淋巴细胞染色体畸变率、微核率和HPRT基因突变率作为中子辐射的生物剂量计的可能性。方法采用14MeV中子等剂量率照射离体人外周血,吸收剂量范围0~1Gy,秋水仙素阻断法检测染色体畸变率,CB细胞法和常规培养法检测微核率,多核细胞法检测HPRT基因突变率,建立相应的剂量效应曲线,比较各指标间的相关性。结果在该剂量范围内,各指标与剂量关系符合现行平方模型,染色体畸变、微核呈过离散分布,三者有线性相关性。染色体畸变率灵敏度最高,其次是CB细胞法微核率,再次是常规培养法微核率,HPRT基因突变率最低。结论中子辐射事故可采用准确灵敏的染色体畸变率和CB细胞法微核率作为生物剂量计进行辐射种类判定和生物剂量估算。 Objective To study the possibility of using human peripheral blood lymphocytes ehro- mosomal aberration, micronucleus and HPRT gene mutation rates induced by neutron irradiation in vit- ro as biological dosimeter. Methods Forteen MV neutron radiation isodose rate was used to treat hu- man peripheral blood lymphocytes in vitro with a dose range from 0 to 1Gy, Colchicine block,cylosine B (CB) stimulated lymphocyte culture, conventional lymphocyte culture, mullinuclear cell method were used to detect the chromosomal aberration rate,the micronucleus frequency and the HPRT gene mutation rate,reapectively,the corresponding dose-response curves were established.The relativities between any two of these indicators were compared. Results In the range of dose used, the relationship of dose and indicator accorded with the current square model. The chromosome aberration rates and micronucleus frequencies presented over discrete distribution.There existed linear correlations among three indicators of them,the chromosome aberration rate was the most sensitive one, followed by CB cell micronucleus frequency, conventional culture micronucleus frequency and HPRT gene mutation rate indiscent order. Conclusion In Neutron accidents,the sensitive and accurate chromosome aberration rate and CB cul- ture micronucleus frequency can be used as biological dosimeter to determine the type of radiation and to estimate its biological dose.
作者 唐大川 杨占山 杨淑琴 TANG Da-chttan, YANG Zhan-shan, YANG Shu-qin (Dept of Radiation Toxicology, School of Radiation Medicine and Public Health, Soochow University, Jiangsu Suzhou 215123, China)
出处 《苏州大学学报:医学版》 CAS 北大核心 2009年第4期660-663,共4页 Suzhou University Journal of Medical Science
基金 国家自然科学基金资助项目(30570549)
关键词 中子辐射 染色体畸变率 微核率 HPRT基因突变率 neutron radiation the chromosomal aberrations rate micronucleus frequency HPRT gene mutation rate
作者简介 作者简介:唐大川(1980-),男,四川南充人,医学硕士,研究方向为辐射生物计量计。 通讯作者:杨占山
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参考文献7

  • 1Tsoulou E,Kalfas CA,Sideris EG. Conformational properties of DNA after exposure to gamma rays and neutrons[J]. Radiation Research,2005,163(1):90-97. 被引量:1
  • 2Guzman-Rincon J, Delfin-Loya A, Urena-Nunez F, et al. Genotoxicity of neutrons in Drosophila melanogaster, somatic mutation and recombination induced by reactor neutrons[J].Radiation Research,2005,164(2): 157-162. 被引量:1
  • 3白玉书,陈德清主编..人类辐射细胞遗传学[M].北京:人民卫生出版社,2006:226.
  • 4IAEA Technical Report Series No. 206. Biological dosimetry: chromosone aberration analysis for dose assessment[S]. Vienna:IAEA,1986:28. 被引量:1
  • 5WS/T187-1999.《淋巴细胞微核估算剂量的方法》[S].北京:中国标准出版社,2000:711. 被引量:1
  • 6Cao J, Sun H, Cheng G. Chromosomal aberration, DNA strand breaks and gene mutations in nasopharyngeal cancer patients unddergoing radiation therapy [J]. Mutai Res 2002,504(1-2):85-89. 被引量:1
  • 7白玉书,黄绮龙.快中子照射离体血建立淋巴细胞微核的剂量效应曲线[J].中华放射医学与防护杂志,1998,18(5):354-356. 被引量:4

二级参考文献4

  • 1白玉书,中华放射医学与防护杂志,1996年,16卷,326页 被引量:1
  • 2白玉书,中华放射医学与防护杂志,1995年,15卷,75页 被引量:1
  • 3蒋本荣,上海"6.25"60Co源辐射事故病人诊断与救治文集,1994年,33页 被引量:1
  • 4关树荣,遗传,1992年,14卷,29页 被引量:1

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