Objective To study the effect of Simiaoyongantang on human umbilical vein endothelial cells （HUVECs） injury induced by hypoxia. Methods The HUVECs were divided into normal group, model group and Simiaoyongantang group, and the hypoxia model was established at the latter two groups. The livability of HUVECs was measured by MTT assay. The transudation of LDH in the medium was measured by biochemistry and the positive expression of VEGF was detected by immunofuorescence chemistry. Results The level of LDH was reduced significantly and the cell activity indicated by MTT was increased. The number of the cells stained by VEGF increased in the treatment group of Simiaoyongantang. Conclution Simiaoyongantang has protective effect on hypoxia injured HUVECs, which may be related to its effect of increasing the cells livability, reducing the injury induced by hypoxia, up-regulating expressions of VEGF and promoting angiogenesis.
Chinese Journal of Information on Traditional Chinese Medicine
human umbilical vein endothelial cells： angiogenesis