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紫甘薯京薯6号脱毒苗的诱导与快繁 预览 被引量:10

Induction and Rapid Propagation of Virus-free Seedlings of Purple Sweet Potatoes Jingchu-6
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摘要 针对紫甘薯京薯6号生产现状,应用植物茎尖分生组织脱毒培养技术,对紫甘薯京薯6号脱毒苗的生产进行研究。结果显示,茎尖分生组织在添加0.75 mg/L 6-BA+0.1 mg/L NAA的MS培养基上可诱导得到无毒苗,出芽率达到100%,出芽平均时间为28d,脱毒率达到95%。以紫甘薯京薯6号脱毒苗茎节为材料,在MS培养基中培养20~25 d可以获得无毒快繁苗,繁殖系数为5~6倍。 Based on its production situation of purple sweet potatoes Jingchu-6,some stem tips were used to induce virus-free seedlings by tissue culture in MS culture medium containing different 6-BA/NAA ratios.The results showed that the induction rate of the virus-free seedlings from the meristems of purple sweet potatoes Jinchu-6 reached 100 percent in MS medium containing 0.75 mg/L 6-BA and 0.1 mg/L NAA with a mean seedling time of 28 days,and the average rate of virus elimination was up to 95 percent.The reproductive cycle of the virus-free seedlings of purple sweet potatoes Jingchu-6 was within 20-25 days in the MS medium mentioned above,and its reproductive coefficient could reach 5-6 times.
作者 段玉云 王新良 金月嵘 侯思名 罗红梅 曾黎琼 DUAN Yu-yun,WANG Xin-liang,JIN Yue-rong,HOU Si-ming,LUO Hong-mei,ZENG Li-qiong,(1.Yunnan Academy of Agricultural Sciences,Yunnan Kunming 650231,China;2.Biotechnology and Genetic Germplasm Institute,Yunnan Academy of Agricultural Sciences,Yunnan Kunming 650223,China;3.College of Life Science and Chemistry,Dali University,Yunnan Dali 671000,China;4.College of Landscape and Horticulture,Yunnan Agricultural University,Yunnan Kunming 650201,China;5.Key Laboratory of Agricultural Biotechnology of Yunnan Province,Yunnan Kunming 650223,China)
出处 《西南农业学报》 CSCD 北大核心 2011年第2期 835-837,共3页 Southwest China Journal of Agricultural Sciences
基金 昆明绿色经济示范区项目([2008]004号)
关键词 紫甘薯京薯6号 脱毒苗 组织培养 快繁 Purple sweet potato Jinchu-6 Virus-free seedling Tissue culture Rapid propagation
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