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红桂木凝集素对Jurkat T淋巴细胞生长的影响 预览 被引量:9

Effects of Artocarpus Lingnanensis Lectin on Proliferation of Jurkat T Lymphocytes
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摘要 目的研究红桂木凝集素(ALL)对Jurkat T淋巴细胞生长的影响。方法将ALL单独作用于CD4+T淋巴细胞系Jurkat E6-1细胞株,WST-8比色法检测细胞增殖;倒置显微镜观察细胞生长变化;酶联免疫夹心法(ELISA)检测IL-2、IL-10和IFN-γ的分泌水平。结果 ALL(0.36~360μg/ml)单独作用后,能够抑制T细胞的增殖,抑制率为1.82%~59.17%(P〈0.01),且呈剂量-效应关系,其半数细胞抑制剂浓度(IC50值)为59.72μg/ml,且镜下可见细胞集落形成受到抑制;ALL对细胞因子IL-2和IFN-γ的分泌均有明显促进作用,ALL(1.8μg/ml)处理组IL-2和IFN-γ的分泌与空白对照组比较分别增加了11.69倍和21.29倍(P〈0.01)。结论 ALL能有效抑制Jurkat T淋巴细胞的生长,且具有剂量-效应关系。 Objective To study Artocarpus lingnanensis lectin(ALL)on the growth of acute lymphoblastic leukemia Jurkat T lymphocytes in vitro.Methods Jurkat cells were incubated with different concentrations of ALL.The proliferation of Jurkat T lymphocytes was analyzed by using WST-8 colorimetric assay and inverted Microscope.The level of IL-2,IL-10 and IFN-γ in the supernatants was measured by enzyme linked immune sorbent assay(ELISA).Results ALL concentration in the range of 0.36-360 μg/ml inhibited the proliferation of Jurkat cells,showing dose-response relationship.Inhibition rates were in the range of 1.82%-59.17%(P0.01),and its IC50 was 59.72 μg/ml.It was also observed that the colony formation of Jurkat T cells decreased along with the increase of ALL concentration.The secretion of IL-2 and IFN-γ by Jurkat cells were enhanced.Compared with the control group,the level of IL-2 and IFN-γ were 11.69 times and 21.29 times higher respectively in ALL(1.8 μg/ml)treatment group(P0.01).Conclusion ALL can suppress Jurkat T lymphocytes proliferation,showing a dose effect relationship.
作者 崔博 曾麒燕 CUI Bo,ZENG Qi-yan(Department of Biochemistry and Molecular Biology,Basic Medical School of Guangxi Medical University,Nanning 530021,China)
出处 《广西医学》 CAS 2011年第4期 410-413,共4页 Guangxi Medical Journal
基金 广西科学基金(0832130)
关键词 红桂木凝集素 JURKAT T淋巴细胞 增殖 细胞因子 Artocarpus lingnanensis lectin Jurkat T lymphocytes Proliferation Cytokine
作者简介 通信作者:曾麒燕,E—mail:zengnn6@hotmail.com
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