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益智仁挥发油抗帕金森模型小鼠黑质神经元凋亡的作用研究 被引量:10

Effects of Volatile Oil Extracted from Alpinia oxyphylla on the Apoptosis of Substantial Nigra Neuron in PD Mice Model
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摘要 目的:研究益智仁挥发油对帕金森(PD)模型小鼠黑质致密部尼氏小体、酪氨酸羟化酶(TH)表达的影响和神经元凋亡的对抗作用。方法:实验分为6组,即正常对照(等容生理盐水)、模型(等容生理盐水,ig生理盐水第6天起同时复制模型,连续7d,每天1次)、司来吉兰(10mg·kg,ig司来吉兰第4天起同时复制模型,连续7d,每天1次)和益智仁挥发油高、中、低剂量(2.5、0.833、0.278mL·kg-1,ig益智仁挥发油第6天起同时复制模型,连续7d,每天1次)组。ip1-甲基-4-苯基.1,2,3,6-四氢吡啶(MPTP)复制C57BL小鼠PD模型。采用尼氏染色法观察尼氏小体表达;SABC免疫组化染色法观察益智仁挥发油对TH表达的影响;TUNEL染色法检测黑质神经元凋亡。结果:与正常对照组比较,模型组小鼠黑质神经元的尼氏小体、TH表达阳性细胞数明显减少,神经元凋亡显著增加;与模型组比较,益智仁挥发油高、中剂量组小鼠黑质神经元内尼氏小体显著增多;益知仁挥发油高、中、低剂量组TH表达阳性细胞数显著增多,而凋亡细胞数量显著减少。结论:益智仁挥发油能对抗PD模型小鼠黑质神经元细胞凋亡。 OBJECTIVE: To study the effect of volatile oil extracted from Alpinia oxyphylla on the expression of Nissl' s cyto- ryctes and TH and the apoptosis of substantial nigra neuron in Parkinson' s disease (PD) mice model. METHODS: Model mice were divided into 6 groups, i.e. normal control group (constant volume normal saline), model group (constant volume normal sa- line, model induced by intragastrical administration of normal saline once a day since 6th day for consecutive 7 days), selegiline group (10 mg.kg1, model induced by intragastrical administration of selegiline once a day since 4th day for consecutive 7 days), A. oxyphylla volatile oil high-dose, medium-dose and low-dose groups (2.5, 0.833, 0.278 mL-kg-1,model induced by intragastrical administration ofA. oxyphylla volatile oil once a day since 6th day for consecutive 7 days). MPTP was used to establish C57BL PD mice model. Nissl staining method was used to detect Nissl cytoryctes expression; SABC staining method was used to detect TH expression; TUNEL staining method was used to detect the apoptosis of substantial nigra neuron in mice. RESULTS: Compared with normal control group, Nissl' s cytoryctes and TH expression of substantia nigra neurons in model group were significantly reduced, and the apoptosis of substantia nigra neurons in model mice were significantly increased; compared with model group, Nissl's cyto- ryctes of PD mice in A. oxyphylla volatile oil high-dose and medium-dose groups increased significantly;TH expression of PD mice in A. oxyphylla voletileoil high-dose, medium-dose, low-dose groups increased significantly, while the apoptosis of substantia nigra neurons were significantly reduced. CONCLUSION: It is suggested that A. oxyphylla volatile oil can relieve the apoptosis of substantia nigra neurons in PD mice.
作者 黄凌 朱毅 邝少轶 HUANG Ling, KUANG Shao-yi(Pharmacology Teaching and Research Section, School of Pharmaceutical Sci- ences, Hainan Medical University, Haikou 571101, China) ZHU Yi(Hainan Institute for Drug Control, Haikou 570216, China)
出处 《中国药房》 CAS CSCD 北大核心 2011年第47期 4430-4433,共4页 China Pharmacy
关键词 益智仁 帕金森病 抗凋亡 Alpinia oxyphylla Parkinson' s disease Anti-apoptosis
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