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补肾化痰复方颗粒对孕期高雄激素血症大鼠雌性子代生殖内分泌的影响 被引量:6

Effects of Bushen Huatan Compound Particle on reproduction endocrinology of PNA rats' female offspring
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摘要 目的:研究补肾化痰复方颗粒对孕期高雄激素血症(PNA)大鼠雌性子代生殖方面的影响并探讨其机制。方法:选取Wistar雌性大鼠,在孕中期颈背部皮下注射丙酸睾丸酮,连续3d,建立PNA大鼠模型。以其雌性子代生长至成年时,用补肾化痰复方连续灌胃20d,观察其动情周期,检测血清雌二醇(E2),睾酮(T),孕酮(P),17羟孕酮(17-OHP)水平,观察卵巢形态学变化,并用RT-PCR技术检测卵巢组织中细胞色素p450 17α羟化酶(cyp17)、细胞色素p450(cyp19)及类固醇激素急性调节蛋白(star)基因的表达。结果:①PNA雌性子代存在无排卵、高雄激素血症表现,卵巢呈多囊改变;②补肾化痰复方能够改善模型鼠动情周期,降低T、P、17-OHP的水平(P〈0.05);③模型组卵巢组织cyp17、cyp19、star均高表达,但cyp17及star经补肾化痰复方治疗后降低(P〈0.05)。结论:①PNA大鼠的子代是研究多囊卵巢综合征(PCOS)的理想模型;②补肾化痰复方能够降低雄激素,逆转卵巢的多囊改变,改善排卵功能,能够调节PCOS模型动物生殖异常情况。 Objective: To study the effects of Bushen Huatan compound particles on androgen synthesis for the prenatally androgenized female rats.Methods:Pregnant rats were treated with daily subcutaneous injections of testosterone propionate from embryonic days 16 to 19,and female offspring were studied as subject.The rats were treated with Bushen Huatan compound particle for 20 days,the improving of estrogen cycle was observed,and the level of estradiol(E2),testosterone(T),progesterone(P),as well as 17a-hydroxyprogestrone(17-OHP) were measured by radioimmunoassay,the changes of ovarian morphological and cellular expression key signal molecules were analyzed by RT-PCR technology.Results: ①the prenatally androgenized female rats were ideal model.② Bushen Huatan compound particle can improve estrogen period lower lever of T,P,17-OHP(P0.05) of model rat.③RT-PCR results showed that cyp17,cyp19 and star are high expression in ovarian of model rat,expression of cyp17,star(P0.05) and cyp19 were lowerd when treated by Bushen Huatan compound particle.Conclusion: ① The prenatally androgenized female rats were ideal model with PCOS;② Bushen Huatan compound particls can reduce androgen,promote the ovulation and improve ovarian function as well as regulative reproduction of PCOS model animals.
作者 常惠 匡洪影 丛晶 梁晨 侯丽辉 吴效科 CHANG Hui,KUANG Hong-ying,CONG Jing,LIANG Chen,HOU Li-hui,WU Xiao-ke(Laboratory of Gynecology,First Af?liated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处 《中华中医药杂志》 CAS CSCD 北大核心 2011年第12期 2872-2875,共4页 China Journal of Traditional Chinese Medicine and Pharmacy
基金 国家中医临床基地(月经病)建设项目[No.国中医药发(2008)23号] 黑龙江省博士后科研启动金(No.LBH-Q09009)
关键词 “补肾化痰”复方 多囊卵巢综合征 孕期高雄激素血症 生殖内分泌 Bushen Huatan Compound Particle PCOS PNA Reproductive endocrinology
作者简介 通讯作者:吴效科,哈尔滨市香坊区和平路24号黑龙江中医药大学附属第一医院妇科实验室,邮编:150040,电话:0451—82130094E—mail:xiaokewu2002@vip.sina.com
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