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hprt基因突变对宫颈癌放疗损伤的评估 预览 被引量:1

Assessment of radiotherapy damage using hprt gene mutation in cervical cancer patients
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摘要 目的:探讨hprt基因突变对宫颈癌放疗损伤的评估作用。方法:取15例宫颈鳞癌放疗患者放疗前及放疗累积剂量为10~60Gy时的外周静脉血,采用多核细胞法检测hprt基因突变率。结果:在照射累积剂量为30、40和50Gy时,hprt基因突变率在照射后升高,与照射前相比,差别有统计学意义(P〈0.05)。当累积剂量达60 Gy时,hprt基因突变率虽然较照射前升高,但差别无统计学意义(P〉0.05)。在0~40 Gy,hprt基因突变率基本呈线形上升,且突变率与累积剂量间呈现较好的剂量-效应关系(r=0.900,P〈0.05)。回归方程为y=0.459+0.014x。结论:在累积剂量0~40 Gy范围内,hprt基因突变率在放疗后明显升高,呈现良好的剂量-效应关系,可用于评价放疗造成的损伤,是具有应用潜质的生物剂量计。 OBJECTIVE:To assess the radiotherapy damage using hprt gene mutation in cervical cancer patients.METHOD:Blood samples were collected from 15 cervical cancer patients exposed to radiation at the accumulated dose of 0-60 Gy.hprt gene mutation was studied by multinucleated cell assay.RESULT:Mutation frequency of hprt gene increased after radiation.The frequency increased significantly at dose 30,40 and 50 Gy compared to 0 Gy(P〈0.05).At the accumulated dose of 60 Gy,the mutation frequency was also higher than 0 Gy,but not statistically significant.The increase of hprt gene mutation frequency was linear from 0-40 Gy.There was a good dose-response effect between mutation frequency of hprt gene and accumulated dose(r=0.900,P〈0.05).Analysis showed a linear dose-response with an equation of y=0.459+0.014x(r=0.900,P〈0.05).CONCLUSION:Mutation frequency of hprt gene increased after radiation from 0-40 Gy.There was a good dose-response relationship between mutation frequency and accumulated dose.Mutation frequency of hprt gene could be a valuable biodosimeter.
作者 武丽蕊 王兰朋 李红霞 王德华 孙莎 WU Li-rui,WANG Lan-peng,LI Hong-xia, WANG De-hua,SUN Sha (1.Department of Gynecology and Obstetrics,China National Petroleum Company Central Hospital,Langfang 065000,Hebei; 2.Department of the Thoracic Surgery,The People's Hospital Lang Fang Langfang 065000,Hebei;3.Department of Gynecology and Obstetrics,General Hospital of Tianjin Medical University,Tianjin 300070,China)
出处 《癌变.畸变.突变》 CAS CSCD 2011年第6期 465-467,共3页 Carcinogenesis,Teratogenesis and Mutagenesis
关键词 宫颈癌 hprt基因位点突变 放疗损伤 剂量-效应关系 cervical cancer hprt gene mutation radiation-induced damage dose-response relationship
作者简介 武丽蕊(1978-),女,河北人,硕士研究生,主治医师,Tel:13785589076;E—mail:wulirui2008@163.com
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