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影响检测HPRT基因突变率的因素探讨 预览 被引量:1

Study on influencing factors for detecting genetic mutation rate of HPRT
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摘要 恶性肿瘤的发生、发展与突变事件密切相关,检出人类在环境中接触的突变剂依赖于致突变试验。以次黄嘌呤鸟嘌呤磷酸核糖基转移酶(hypoxanthine-guanine phosphoribosyl transferase,HPRT)基因为报告基因的HPRT突变试验就是其中几个重要的致突变试验之一,由于其本身所具有的独特生物学特性,逐渐成为基因突变机制和修复机制理想的研究靶点,HPRT基因突变分析法也逐渐成为很有价值并被广泛应用的生物剂量计。但是,HPRT基因突变率的检测也受诸多因素影响,例如高剂量时,射线造成的已不仅仅是单基因损伤,另外,HPRT自发突变频率受年龄、样本数量等的影响。因此,HPRT基因突变频率已不能完全反映损伤程度与剂量之间的关系。 The occurrence and development of malignant tumors are closely related with the mutation events.Detect-ing mutagens exposure to the human environment is dependent on the mutagenicity test.Using hypoxanthine guanine phospho-ribosyl transferase(HPRT) gene as a reporter gene,the HPRT mutation test is one of the few important mutagenicity tests.Due to its unique biological characteristics,the HPRT test gradually has become the ideal study target of genetic mutation mecha-nism and repair mechanism.The HPRT gene mutation analysis has gradually become a very valuable and widely used biologi-cal dosimeter.However,the HPRT gene mutation detection is affected by many factors,such as high doses,ray causes not only single genetic damage,in addition the HPRT spontaneous mutation frequency is affected by age,number of samples and so on.Therefore,the mutations frequency of HPRT can not completely reflect the relationship between degree of injury and dose.
作者 刘波(综述) 梁庆模(审校) LIU Bo,LIANG Qing-mo(Department of Science and Education,Affiliated Nanhua Hospital,Nanhua University,Hengyang,Hunan 421002,China)
出处 《现代医药卫生》 2012年第1期 87-88,共2页 Modern Medicine Health
关键词 辐射 HPRT基因突变 剂量 Radiation HPRT genetic mutation Dose
作者简介 通讯作者:梁庆模(E—mail:hylqm@vip.sina.com)。
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