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HPLC测定左归丸中齐墩果酸与熊果酸含量 被引量:10

Determination of Ursolic Acid and Oleanolic Acid in Zuogui Wan by HPLC
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摘要 目的:建立HPLC测定左归丸中齐墩果酸、熊果酸含量的方法。方法:采用反相高效液相色潽法,Sunfire C18柱,甲醇-水(85∶15)(磷酸二氢钠调pH 6.5),流速1.0 mL.min-1,检测波长215 nm,柱温25℃。结果:齐墩果酸在0.05~1.0μg线性关系良好(r=0.999 9),回收率97.7%(RSD 1.39%,n=6);熊果酸在0.1~2.0μg线性关系良好(r=0.999 9),回收率98.3%(RSD 0.82,n=6)。结论:方法可用于左归丸中熊果酸、齐墩果酸的含量测定。 Objective:To establish a HPLC method for the determination of ursolic acid and oleanolic acid in Zuogui Wan.Method: The method was applied with Sunfire C18 column by methanol-water(25∶75,adjust pH to 6.5 with sodium dihydrogen phosphate) as the mobile phase,with the flow rate 1.0 mL·min-1.The column temperature was maintained at 25 ℃ and the UV detection wavelength was set at 215 nm.Result: Oleanolic acid was linear in the range of 0.05-1.0 μg(r=0.999 9),the average recovery was 97.7%(RSD 1.39,n=6).Ursolic acid was linear in the range of 0.1-2.0 μg(r=0.999 9),the average recovery was 98.3%(RSD 0.82,n=6).Conclusion: The method can be used for determination of oleanolic acid and ursolic acid in Zuogui Wan.
作者 张汶婕 管大平 钱世兵 ZHANG Wen-jie,GUAN Da-ping,QIAN Shi-bing(Tongling Institute for Food and Drug Control,Tongling 244000,China)
出处 《中国实验方剂学杂志》 CAS 北大核心 2012年第5期 92-94,共3页 China Journal of Experimental Traditional Medical Formulae
关键词 左归丸 齐墩果酸 熊果酸 高效液相色谱法 含量测定 Zuogui Wan ursolic acid oleanolic acid HPLC content
作者简介 [通讯作者]张汶婕,硕士学位,主管药师,从事中药检验工作,Tel:05622831625,E-mail:zhangwenjie85@163.cam
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