Aims The objective of our research is to explore the consistency between traditional culture and real-time quantitative PCR（qPCR） through testing fecal microbiota in patients with liver cirrhosis. Methods 73 patients with liver cirrhosis and 55 healthy controls were enrolled. Their fecal bacteria were detected by traditional culture and qPCR, and the results from two different methods were compared. Results To compare with healthy controls, culture results showed that the lever of Escherichia coli, Enterococcus, staphylococcus and yeast was significantly enriched（P = 0.004, 0.002, 0.044, 0.026, respectively）, Bacteroides, LactobaciUus, Bifidobacterium and Clostridium were significantly reduced in patient group（P = 0.029, 0.046, 0.045, 0.045, respectively）, qPCR results suggested that the amount of Escherichia coli and Enterococcus were statistically increased（P 〈 0.01, 0.012, respectively）, Lactobacillus, Bifidobacterium, Bacteroides and Clostridium were statistically decreased（all P 〈 0.01）. Conclusion Fecal microbial communities are imbalanced in cirrhotic pa- tients compared with healthy individuals, which may affect the prognosis. The results of traditional culture and qPCR are consistent, but the latter with the more accurate and sensitive, time saving, energy saving.
Modern Digestion ＆ Intervention
Real-time quantitative PCR