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两种方法定量分析肝硬化患者肠道菌群改变的比较 预览 被引量:3

Comparison of two methods applied in the quantitative analysis of gut flora alteration in patients with liver cirrhosis
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摘要 目的观察肝硬化患者肠道菌群改变,探讨传统培养法与实时定量PCR检测结果的一致性。方法纳入73名肝硬化患者及55名健康人,对其粪便细菌进行传统培养及定量PCR,比较两种方法检测结果的一致性。结果细菌培养结果显示,与正常对照组比较,肝硬化患者肠杆菌、肠球菌、葡萄球菌、酵母菌数量显著增加(P分别为0.004、0.002、0.044、0.026),拟杆菌、乳酸杆菌、双歧杆菌、梭菌数量显著降低(P分别为0.029、0.046、0.045、0.045)。定量PCR结果显示,肝硬化组肠杆菌、肠球菌数量显著增高(P分别〈0.01、0.012),乳酸杆菌、双歧杆菌、拟杆菌及梭菌数量显著降低(P均为〈0.01)。结论肝硬化患者存在不同程度肠道菌群失衡,可能影响病情发展。传统细菌培养与荧光定量PCR的结果具有良好的一致性,但定量PCR更精确、灵敏、省时、省力。 Aims The objective of our research is to explore the consistency between traditional culture and real-time quantitative PCR(qPCR) through testing fecal microbiota in patients with liver cirrhosis. Methods 73 patients with liver cirrhosis and 55 healthy controls were enrolled. Their fecal bacteria were detected by traditional culture and qPCR, and the results from two different methods were compared. Results To compare with healthy controls, culture results showed that the lever of Escherichia coli, Enterococcus, staphylococcus and yeast was significantly enriched(P = 0.004, 0.002, 0.044, 0.026, respectively), Bacteroides, LactobaciUus, Bifidobacterium and Clostridium were significantly reduced in patient group(P = 0.029, 0.046, 0.045, 0.045, respectively), qPCR results suggested that the amount of Escherichia coli and Enterococcus were statistically increased(P 〈 0.01, 0.012, respectively), Lactobacillus, Bifidobacterium, Bacteroides and Clostridium were statistically decreased(all P 〈 0.01). Conclusion Fecal microbial communities are imbalanced in cirrhotic pa- tients compared with healthy individuals, which may affect the prognosis. The results of traditional culture and qPCR are consistent, but the latter with the more accurate and sensitive, time saving, energy saving.
作者 唐源淋 张绍衡 郭亚兵 姜泊 陈烨 TANG Yuan-lin, ZHANG Shao-heng, GUO Ya-bing, JANG Bo, CHEN Ye. Guangdong Provincial Key laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern medical University, Guangzhou 510515, China.
出处 《现代消化及介入诊疗》 2012年第1期 5-8,共4页 Modern Digestion & Intervention
基金 广东省自然科学基金(9151051501000048)
关键词 肠道菌群 肝硬化 传统培养 实时定量PCR Gut flora Liver cirrhosis Traditional culture Real-time quantitative PCR
作者简介 通讯作者:陈烨,E-mail:Chenye2001@hotmail.com
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参考文献19

  • 1Garcia-Tsao G,Wiest R.Gut microflora in the pathogenesis of the complications of cirrhosis.Best Pract Res Clin Gastroenterol,2004,18:353-372. 被引量:1
  • 2范东旭,杨婷,房淑清,罗维,王跃生.肝硬化病人血清内毒素水平与肠道菌群的关系[J].黑龙江医药科学,2005,28(1):12-14. 被引量:6
  • 3陈穗,陈桂明.肝炎后肝硬化患者肠道菌群变化初步分析[J].中华消化杂志,1995,15(2):75-77. 被引量:45
  • 4Matsuki T,Watanabe K,Fujimoto J,et al.Development of16S rRNA-Gene-Targeted group-specific primers for the detection and identification of predominant bac. 被引量:1
  • 5Rinttil,T,Kassinen A,Malinen E,et al.Development of an exten-sive set of16S rDNA targeted primers for quantification of pathogenic and indigenous bacteri. 被引量:1
  • 6Eckburg PB,Bik EM,Bernstein CN,et al.Diversity of the human intestinal microbial flora.Science,2005,308(5728):1635-1638. 被引量:1
  • 7McKenna P,Hoffmann C,Minkah N,et al.The macaque gut micro-biome in health,lentiviral infection,and chronic enterocolitis.PLoS Pathog,2008,4(2):e20. 被引量:1
  • 8Dethlefsen L,Huse S,Sogin ML,et al.The pervasive effects of an antibiotic on the human gut microbiota,as revealed by deep16S rRNA sequencing.PLoS Biol,20. 被引量:1
  • 9Turnbaugh PJ,Hamady M,Yatsunenko T,et al.A core gut micro-biome in obese and lean twins.Nature,2009,457(7228):480-484. 被引量:1
  • 10Bajzer M,Seeley RJ.Physiology:obesity and gut flora.Nature,2006,444(7122):1009-1010. 被引量:1

二级参考文献10

共引文献48

同被引文献24

  • 1金峰,张捷,张志坚,余英豪,李鸣,袁芳,薛建设.双歧杆菌制剂对大鼠肝硬化模型的影响[J].中国微生态学杂志,2004,16(5):269-271. 被引量:2
  • 2黄自平 粱扩寰.肝病时的内毒素血症[A].粱扩寰 主编.肝脏病学 第1版[C].北京,人民卫生出版社,1995.397—411. 被引量:8
  • 3Madrid A M,Brahm J,Antezana C,et al.Small bowel motility inprimary biliary cirrhosis[J].Am J Gastroenterol,1998,93(12):2436-2440. 被引量:1
  • 4Kim J W,Jeon W K,Yun J W,et al.Intestinal permeability in pa-tients with viral and alcoholic liver disease[J].Korean J Gastroen-terol,2004,43(2):104-111. 被引量:1
  • 5Guarner C,Soriano G.Bacterial translocation and its consequencesin patients with cirrhosis[J].Eur J Gastroenterol Hepatol,2005,17(1):27-31. 被引量:1
  • 6Vlachogiannakos J,Daikos G,Thalheimer U,et al.Is bacterial DNAa better marker than endotoxin of bacterial translocation in decom-pensated cirrhosis[J].Hepatology,2011,53(6):2140-2141. 被引量:1
  • 7Xing HC, Li LJ, Xu KJ. Protective role of supplement with foreign Bifidobaeterium and Laetobaeillus in experimental hepatic isehemia- reperfusion injury[ J]. J Gastmenteml Hepatol,2006 ,21:647456. 被引量:1
  • 8Meng QH, Yu HW, Li J, Inadequate nutritional in take and protein- energy malnutrition involved in acute and chronic viral hepatitis Chi- nese patients especially in cirrhosis atients [ J ]. Hepatogastroenterol- ogy,2010,57( 101 ) :845-851. 被引量:1
  • 9Marcellin P, Gane E, Buti M. Regression of cirrhosis during treatment with tenofovir disoproxil fumarate for chronic hepatitis B : a 5-year open-label follow-up study [ J ]. Lancet, 2012,11 : 1-8. 被引量:1
  • 10Othman M, Aguero Pt ,Lin HC. Alterations in intestinal microbial flo- ra and human disease [ J ]. Curr Opin Gastroenterol, 2008,24 : 11 - 16. 被引量:1

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