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5种枣果实中凝集素的提取及生物活性分析 预览 被引量:4

Extraction and Biological Activity Analysis of Lectin from Five Cultivars of Chinese Jujube
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摘要 提取5种枣果实(壶瓶枣、骏枣、黄河滩枣、黑叶子枣、大白铃枣)的凝集素,经硫酸铵沉淀、透析,得到蛋白质提取液,用不同类型的人血细胞(O型、A型、B型、AB型)检测其凝集活性。通过凝集活性和热稳定性实验比较5种枣果实凝集素的生物活性。结果表明:各个品种的枣果实的凝集素对不同血型均有不同程度的凝集效果,如壶瓶枣、黄河滩枣和大白铃枣对A型血的凝集效价都可以达到2^7,而骏枣和黑叶子枣略有差异,分别达到2^5和2^6,黄河滩枣、黑叶子枣和大白铃枣对B型血的凝集效价可以达到2^7,骏枣和壶瓶枣略有差异,分别达到2^5和2^6;5种枣果实凝集素均能耐70℃高温,在80℃以后均失去凝集活性;5种枣均无单糖和寡糖的专一结合位点,而且5种枣果凝集素活性的维持与金属离子Mg^2+、K^+、Cu^2+、Fe^3+的关系不大。 Lectin from 5 culfivars of Chinese jujube was isolated by ammonium precipitation and dialysis. Its ability to induce blood cell aggregation was then evaluated using different blood types (A, B and O). The results obtained showed that lectin from different cultivars of Chinese jujube had different aggregation-inducing activities for different blood types. For example, the blood cell aggregation valence to blood type A of lectin from Chinese jujube cultivars Huping, HuangHetan and Dabailing reached 2^7 compared with 2^5 and 2^6 for lectin from Chinese jujube cultivars Jun and Huping dates, respectively. Lectin from five different Chinese jujube cultivars were all stable at 70℃. However, when the temperature reached 80 ℃, all of them lose aggregation-inducing ability. Moreover, they had no monosaccharide and oligosaccharide specific binding sites. The metal ions Mg^2+, K^-, Cu^2+ and Fe^3+ were not essential for its blood cell aggregation activity.
作者 韩红艳 冀爱青 刘林敬 申奥 HAN Hong-yan, JI Ai-qing, LIU Lin-jing, SHEN Ao (l.College of Biological Science and Biotechnology, Jinzhong University, Yuci 2. College of Life Science, Tianjin Normal University, Tianjin 300387,China )
出处 《食品科学》 CAS CSCD 北大核心 2012年第7期 270-274,共5页 Food Science
关键词 枣果实 凝集素 血细胞 生物活性 jujube fruit lectin blood cells biological activity
作者简介 韩红艳(1976-),女,讲师,硕士,研究方向为果品贮藏与细胞生物学。E-ail:hhy0822@126.com
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