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红桂木凝集素对树突状细胞生长的影响 预览 被引量:10

Effects of Artocarpus Lingnanensis Lectin on the Growth of Dendritic Cell
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摘要 目的研究红桂木凝集素(ALL)对人外周血单核细胞来源的树突状细胞生长的影响。方法取健康人外周血分离单个核细胞,分别给予重组人粒细胞-巨噬细胞因子(rhGM-CSF)、重组人白细胞介素-4(rhIL-4)、肿瘤坏死因子-α(TNF-α)和不同浓度的ALL进行培养。用倒置显微镜观察其形态;CCK-8法检测细胞增殖;酶联免疫吸附测定法(ELISA)检测TNF-α、IL-12/P70水平;流式细胞仪检测细胞表型。结果不同浓度的ALL均能显著促进树突状细胞增殖,并促进IL-12/P70和TNF-α分泌,随着浓度的增加增殖率、IL-12/P70和TNF-α水平逐渐提高(P〈0.05)。结论ALL能有效促进人外周血单核细胞来源的树突状细胞的成熟及增殖。 Objective To study artocarpus lingnanensis lectin(ALL) on the growth of human peripheral blood monocyte-derived dendritic cell(DC). Methods The mononuelear cells were obtained from healthy human aseptic fresh peripheral blood, and then the cells were cultured with rhGM-CSF, rhIL-4, TNF-α and different concentrations of ALL, respectively. The morphological features were observed by invert optical microscope. The cell proliferation was analyzed by cell counting kit-8 (CCK-8) assay. The levels of IL-12/P70 and TNF-α in the supematants were determined by enzyme-linked immune sorbent assay (ELISA). The phenotypes changes were evaluated by flow cytometry (FCM). Results ALL could promote not only the proliferation of DC but also the secretion of IL-12/P70 and TNF-α. The cell proliferation and the levels of IL-12/P70 and TNF-α increased in a dose-dependent manner( P 〈 0.05 ). Conclusion ALL can effectively promote the mature and proliferation of human peripheral blood monocyte-derived DC.
作者 王婧娉 李璐 曾麒燕 WANG Jing-ping, LI Lu, ZENG Qi-yan ( Department of Biochemistry and Molecular Biology, Basic Medical College, Gttangxi Medical University, Nanning 530021, China)
出处 《广西医学》 CAS 2012年第6期670-672,共3页 Guangxi Medical Journal
基金 广西自然科学基金(0832130)
关键词 红桂木凝集素 树突状细胞 增殖 细胞因子 Artocarpus lingnanensis lectin Dendritic cell Proliferation Cytokine
作者简介 通信作者:曾麒燕,E-mail:zengnn6@hotmail.com
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